骨纤维异常增殖症病变中骨髓间充质干细胞的分离培养及鉴定  

Isolation and identification of human bone mesenchymal stem cells in fibrous dysplasia of bone in vitro

作　　者：于舰斌[1] 郑培惠[1] 张东升[1] 李胜锋[1] 刘桂军[1] 牟文丽[2] 张捷[2] 

机构地区：[1]山东大学附属省立医院口腔颌面外科,东济南250021 [2]山东大学附属省立医院临床科研中心,东济南250021

出　　处：《中国口腔颌面外科杂志》2011年第2期91-95,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(30973328);上海市重点学科建设项目(S30206)

摘　　要：目的:探索从骨纤维异常增殖症(FD)病变组织中分离、培养骨髓间充质干细胞(MSC)的有效方法,为研究骨纤维异常增殖症的病因及发病机制奠定基础。方法:取FD患者新鲜病变组织,将其剪碎后冲洗,获得骨髓间充质干细胞,进行培养,观察细胞形态,绘制细胞生长曲线,检测其细胞表面标志物及向成骨细胞的诱导分化能力。结果:培养的骨髓干细胞随传代次数增多,细胞形态趋向长梭形,99%的细胞表达CD44和HLA-ABC,不表达CD34;可见诱导后的MSC碱性磷酸酶染色阳性,并形成钙结节。结论:在新鲜FD骨标本中可以成功提取MSC,其在体外具有诱导成骨的能力,获取到的MSC可以满足后续实验的要求。PURPUSE： To investigate a method of isolation and identification of bone mesenchymal stem cells from patients with fibrous dysplasia of bone in vitro and lays the foundation for the study of the etiology and pathogenesis of fibrous dysplasia.METHODS： The fresh bones of patients with FD were obtained.The samples were cut and washed into dish so as to acquire the bone mesenchymal stem cells（MSC）.After cell culture,their morphology was observed,cell growth curve was drawn,and cell surface marker and osteogenic differentiation potency were detected.RESULTS： With the increasing number of MSC passage,the MSC morphology trended to be fusiform shape.99% of the cells expressed CD44 and HLA-ABC,but no expressed CD34.The cells were positive for alkaline phosphatase staining and calcium nodules after being induced.CONCLUSION： MSCs can be successfully obtained from the fresh specimens of FD and can be induced for osteogenesis in vitro.The acquired MSCs can meet the subsequent experimental request.Supported by National Natural Science Foundation of China（30973328） and Shanghai Leading Academic Discipline Project（S30206）.

关 键 词：骨纤维异常增殖症 骨髓间充质干细胞 细胞培养 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]