茶藨生柱锈重寄生木霉粗毒素的基本性质  

Elementary Properties of Crude Toxin Produced by Mycoparasite(Trichoderma atroviride)of Cronartium ribicola

作　　者：李永艳[1] 敖新宇[2] 陈玉惠[2] 

机构地区：[1]西南林业大学保护生物学学院,云南昆明650224 [2]西南林业大学基础部,云南昆明650224

出　　处：《天津农业科学》2011年第2期11-15,共5页Tianjin Agricultural Sciences

基　　金：西南林业大学重点基金项目(110706);云南省森林灾害预警与控制重点实验室资助项目(ZK09A105)

摘　　要：采用液体发酵和生物测定相结合的方法,研究了茶藨生柱锈(Cronartium ribicola)重寄生木霉SS003菌株(Trichoderma atroviride)所产粗毒素的基本性质。结果表明,SS003所产粗毒素为一类非蛋白质、极性较大的物质,具有较高的热稳定性和酸碱稳定性,能被甲醇较好地提取出来,可被活性碳有效地吸附并为甲醇所洗脱。该结果为SS003菌株的活性成分研究及进一步开发利用奠定基础。Adopting the methods of liquid culture and biologic detection,the elementary properties of crude toxin produced by mycoparasite（Trichoderma atroviride） SS003 of Cronartium ribicola were studied.The results showed that the crude toxin produced by SS003 was a kind of non-protein material with higher polarity.The produced toxin,which could be tolerant to high temperature and pH value variations,could be fully extracted by methanol,the toxin was effectively absorbed by the activated charcoal and be eluted by methanol.This result could lay the foundation for the active constituent research and further development usage of SS003 strain.

关 键 词：木霉 粗毒素 基本性质 茶藨生柱锈 

分 类 号：S432.4[农业科学—植物病理学]