苜蓿花药悬浮培养体系的建立  

Establishment of anther suspension culture system for alfalfa

作　　者：姚喜红[1,1] 魏臻武[1,2] 耿小丽[1,1] 杨思维[1,1] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《草原与草坪》2011年第2期53-56,共4页Grassland and Turf

基　　金：国家"863"计划项目(2008AA10Z149);国家自然科学基金项目(30972136);国家科技支撑计划项目(2008BADB3B10)资助

摘　　要：以苜蓿花药愈伤组织为材料建立悬浮细胞系,对悬浮细胞系建立的适宜培养基进行筛选,并对培养液的pH值及细胞活力进行研究。结果表明,参试苜蓿品种在NB液体培养基中愈伤组织诱导率较高;同一激素水平下,NB培养基愈伤组织诱导率明显高于MS液体培养基。培养液pH值出现下降—上升—平稳的趋势。接种第3 d悬浮细胞活力最强。The anther callus of alfalfa was used to establish suspension culture system,and the medium,pH value and cell viability of the system were studied.The result indicated that the callus induction percentage was high in NB liquid medium.At the same level of phytohormones,the callus induction percentage was higher in NB medium than MS liquid medium.The pH value of liquid medium showed a tendency of down-up-stable.The cell viability was the highest in third day.

关 键 词：苜蓿 花药 悬浮培养 培养基 PH值 细胞活力 

分 类 号：S54[农业科学—作物学] S311