免疫透射比浊法测定视黄醇结合蛋白的技术性能评价  

Performance Evaluation of Immune Transmission Turbidity for Retinol Binding Protein

作　　者：蔡晔芬[1] 黄清松[2] 

机构地区：[1]广州医学院附属肿瘤医院,广东广州510095 [2]广东药学院,广东广州510006

出　　处：《宜春学院学报》2011年第4期74-75,共2页Journal of Yichun University

基　　金：2010年广东省医学科学技术研究基金项目(编号:A2010293)

摘　　要：目的:对免疫透射比浊法测定视黄醇结合蛋白进行技术性能评价。方法:根据美国临床实验室标准化委员会评价方案,对免疫透射比浊法测定视黄醇结合蛋白的精密度、线性范围、干扰因素和准确度加以评价。结果:正常值和高值的批内CV%为2.3%和1.7%,批间CV%为3.9%和3.1%,日间CV%为4.5%和3.8%,总CV%为5.5%和4.5%;线性上限可达120 mg/L;胆红素浓度低于442 umol/L和血红蛋白浓度低于5 g/L时对测定无显著干扰(P>0.05);平均回收率为99.2%。结论:免疫透射比浊法测定视黄醇结合蛋白符合临床检验的要求,加上自动化程度高,操作简便,检测速度快,值得临床推广。Objective To evaluate the method of Immune transmission turbidity for retinol binding protein.Methods According to the U.S.Committee for Clinical Laboratory Standards to evaluate the method of Immune transmission turbidity of retinol-binding protein on precision,linear range,interference factor and Accuracy.Results The intra CV% of the Normal and high values are 1.7% to 2.3%,the between CV% are 3.9% to 3.1%;Day CV% was 4.5% to 3.8%;Linear limit of up to 120mg/L.It is no significant interference to determination when bilirubin concentrations below 442 umol/L and hemoglobin concentrations of less than 5g/L（P 0.05）;The average recovery was 99.2%.Conclusions The Immune turbidimetric method for the determination of retinol binding protein consistent with the requirements of clinical laboratory,Because of high degree of automation,easy operation,fast detection,so worthy to be popularized.

关 键 词：免疫透射比浊法 视黄醇结合蛋白 技术性能评价 

分 类 号：R593[医药卫生—内科学]