多菌株混合发酵豆粕的研究  

Study on fermenting soybean meal by a multi-strain mixture

作　　者：王德培[1,2] 管叙龙[1,2] 邓旭衡[3] 魏征[1,2] 丁友昉[1,4] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]工业微生物教育部重点实验室,天津300457 [3]天津市工业微生物重点实验室,天津300457 [4]北京延庆阔利达生物技术有限公司,北京102101

出　　处：《粮食与饲料工业》2011年第4期36-39,共4页Cereal & Feed Industry

摘　　要：利用枯草芽孢杆菌、凝结芽孢杆菌、乳酸杆菌对豆粕进行生料混菌发酵,制备生物活性饲料。通过单因素和响应面分析试验,以三氯乙酸可溶性氮(TCA-NSI)为优化指标对其发酵工艺条件进行研究,得出最佳发酵条件为:枯草芽孢杆菌与乳酸菌之比为2.2∶1、发酵温度30℃、外加水量46.5%、总接种量15 ml(在豆粕72 g、麸皮8 g的发酵基质中)、拌料水pH值自然。在此条件下发酵84 h,测得三氯乙酸可溶性氮含量为12.78%。Raw soybean meal was fermented by the strains mixture of Bacillus subtilis,Bacillus coagulans and Lactobacillus to produce bioactive feed.The fermentation operation conditions were studied via single-factor experiments and response surface analysis and with the content of trichloroacetic acid soluble nitrogen（TCA-NSI） as the optimal index.Finally the optimal fermentation operation parameters were obtained as follows： inoculum ratio of Bacillus bacteria to Lactobacillus 2.2∶1,fermentation temperature 30℃,amount of water added 46.5%,total inoculum dose 15 ml（fermentation matrix of soybean meal 72 g and bran 8 g） and the pH of mixed water was natural.Under those conditions,the TCA-NSI content reached 12.78% after a 84 h fermentation process.

关 键 词：生料混菌发酵 豆粕 饲料 三氯乙酸可溶性氮 枯草芽孢杆菌 乳酸杆菌 

分 类 号：S816.3[农业科学—饲料科学] S816.35[农业科学—畜牧学]