MxA启动子和elF-2a调节区2基因多态性对HBV感染自然转归的影响  

Influences of the gene polymorphisms of MxA promoter and elF-2a-reg2 on the clinical outcome of hepatitis B virus infection

作　　者：魏新素[1] 张平安[1] 李艳[1] 邓斌[2] 

机构地区：[1]武汉大学人民医院检验科,武汉430060 [2]武汉大学人民医院感染科,武汉430060

出　　处：《国际检验医学杂志》2011年第5期536-537,540,共3页International Journal of Laboratory Medicine

基　　金：湖北省自然科学基金资助项目(2008CDB164)

摘　　要：目的探讨干扰素诱导的黏病毒抗性蛋白A(MxA)和真核细胞起始因子2a调节区2(elF-2a-reg2)基因的单核苷酸多态性(SNP)对乙型肝炎病毒(HBV)感染自然转归的影响。方法收集湖北地区160例HBV自限感染者和243例慢性HBV感染者的外周血标本,应用聚合酶链反应-限制性片段长度多态性分析方法,检测MxA启动子-88、-123位点及elF-2a-reg2基因型。结果 MxA启动子-88G/T位点基因型和等位基因在慢性HBV感染者和自限性感染者中的分布差异有统计学意义(P<0.05)。HBV自限性感染者MxA启动子-88位点GG基因型和G等位基因频率分别为41.3%、62.8%,均较慢性感染者频率52.7%、74.9%低(P=0.025和P=0.001),而TT基因型和T等位基因频率分别为15.6%、37.2%,均较慢性感染者频率2.9%、25.1%高(P=0.000和P=0.001),比值比为6.24,95%可信限2.63～14.81。然而,MxA启动子-123位点、elF-2a-reg2基因的不同基因型和等位基因在HBV自限性感染组和慢性感染组间的分布频率差异均无统计学意义(P>0.05)。结论 MxA启动子-88G/T位点多态性与HBV感染后的结局有关,其中TT基因型或T等位基因的存在可能有利于HBV感染后的清除。Objective To explore the influences of the gene polymorphisms of myxovirus resistance A（ MxA） promoter and eukarocyte initiation factor 2 alfa regulatory region 2（elF-2a- reg2） on the outcome of hepatitis B virus（HBV）infection. Methods After the process of extracting genomic DNA from blood of 160 subjects who spontaneously recovered and 243 patients with chronic HBV infection, three single nucleotide polymorphism（SNP）sites in MxA promoter marked as -88G/T,-123C/A and elF-2a-reg2 were determined by polymerase chain reaction（PCR） restriction fragment length polymorphism（RFLP）analysis. Results Subjects with self-limiting infection had a lower frequency of MxA--88 GG genotype（41. 3% vs 52. 7%, P= 0. 025）or G allele（62. 8% vs 74.9 %, P = 0.001 ）, and a higher frequency of TT genotype（15.6 %, vs 2.9 %, P= 0. 000） or T allele（37.2 % vs 25.1 %, P= 0.001） than patients with persistent HBV infection（OR：6.24,95 M CI： 2.63 - 14.81）. However,no significant differences in the distribution of MxA -123C/A and elF-2a-reg2 gene SNP between patients with persistent HBV infection and subjects with self-limiting HBV infection were observed （P 〉 0. 05）. Conclusion There is an association between polymorphisms of the promoter region -88G/T of MxA and the resolution of HBV infection. The presence of the TT genotype or T allele in MxA promoter -88 sites may be resistant to HBV infection,which gives some new clues to the study of pathogenesis of persistent HBV infection.

关 键 词：乙型肝炎病毒 黏病毒抗性蛋白A 真核细胞起始因子2a调节区2 单核苷酸多态性 

分 类 号：R512.62[医药卫生—内科学]