线粒体型天门冬氨酸氨基转移酶在大鼠肝移植术后的变化意义  

Significance of the change in mitochondrial aspartate aminotransferase after rat＇s liver transplantation

作　　者：庄卓男[1] 张培建[1] 孟中良[1] 张杰[1] 苏辉[1] 

机构地区：[1]扬州大学第二临床医学院普通外科研究室,扬州225001

出　　处：《国际外科学杂志》2011年第4期241-244,共4页International Journal of Surgery

基　　金：江苏省卫生厅科研课题（No.H200770）;扬州市科技计划项目（No.YZ2008042）

摘　　要：目的 探讨线粒体型天门冬氨酸氨基转移酶（m-ASI）以及m-AST与天门冬氨酸氨基转移酶（AST）比值在大鼠自体原位肝移植手术中的变化意义.方法 采用大鼠门静脉灌注的自体原位肝移植模型,A组：自体原位肝移植组;B组：正常大鼠假手术对照组.测定两组大鼠术后各时间段血清中m-AST、AST及ALT,计算出m-AST/AST比值,观察其在手术后的变化.结果 术后1 h A组ALT为1149.2 U/L,而B组ALT为111.3 U/L;术后1 h A组AST为819.5 U/L,而B组AST为128.2 U/L;术后1 h A组m-AST为290.8 U/L,而B组m-AST为40.5 U/L.大鼠自体原位肝移植术后m-AST、AST及ALT显著高于正常对照组（P＜0.05）,表明A组与B组相比肝脏损害的程度明显增加.A组m-AST/AST随时间延长其变化较明显.m-AST较AST在血清中半寿期短,而术后AST在本试验中则缓慢恢复正常,致使A组m-AST/AST比值术后6 h明显下降,值为0.12,说明术后6 h大鼠肝细胞线粒体损伤有所恢复.结论 血清m-AST的变化有利于大鼠肝移植手术的预后判断,较AST相比更能在早期反映肝细胞损伤及恢复情况,对大鼠肝移植术后的肝细胞及其线粒体损伤的评估和诊断治疗有指导价值.Objective To investigate the change of the mitochondrial aspartate aminotransferase （mAST）, and the ratio of m-AST and aspartate aminotransferase （AST） in rat orthotopic liver transplantation.Methods We used the rat autologous orthotopic liver transplantation modelin which liver was infused by portal vein. Group A： Autologous orthotopic liver transplantation. Group B： Sham control group of normal rats. To measure the m-AST, AST and ALT in rat serum at each time, the ratio of m-AST/AST was calculated to observe the changes after surgery. Results The ALT of group A after 1 h was 1149.2 U/L, while the ALT of group B was 111.3 U/L; The AST of group A ofter 1 h was 819.5 U/L, while the AST of group B was 128.2 U/L; The m-AST of group After 1 h was 290.8 U/L, while the m-AST of group B was 40.5 U/L. The levels of m-AST, AST and ALT in group A were significantly higher than group B （P 〈 0. 05）.Group A significantly increased the degree of liver damage compared with group B. The ratio of m-AST/AST in group A changed with time obviously. Because the haff-life of m-AST in serum was shorter than that of AST, and AST in this study returned to normal slowly, the ratio of m- AST/AST in A group decreased after 6 h and the number is 0. 12. It indicating that the damage of mitochondria in rat liver cells has been restored after 6h. Conclusions It will be better to judge the prognosis of liver transplantation from the changes of serum m-AST in rats. And it seems earlier to reflect the injury or recovery of liver cell compared with AST.It also has the guiding values to diagnose and treat the damage of liver cells and mitochondrial in the rat liver transplantation.

关 键 词：肝移植 线粒体 线粒体型天门冬氨酸氨基转移酶 

分 类 号：R657.3[医药卫生—外科学]