吉林省粳稻盐胁迫响应差异表型和蛋白质分析  

Analysis on Differential Phenotype and Protein of Rice in Response to Salt Stress in Jilin Province

作　　者：侯军梅[1] 王丹[1] 闫海生[1] 马景勇[1] 

机构地区：[1]吉林农业大学农学院,长春130118

出　　处：《吉林农业科学》2011年第2期1-3,20,共4页Journal of　Jilin Agricultural Sciences

基　　金：吉林省科技支撑项目(20070227);长春市科技支撑项目(09KZ28);吉林省财政厅作物育种项目(zx08001-027B)

摘　　要：以吉林粳稻耐盐型吉8945和盐敏感型吉农大808为试验材料,通过双向聚丙烯酰胺凝胶电泳技术(2-DE)以及基质辅助激光解吸附离子化时间飞行质谱(MALDI-TOF-MS/MS)技术,分别对早期幼苗(10d苗龄)在正常和盐胁迫条件(100 mmol/L NaCl)下盐胁迫响应蛋白质组进行分析、鉴定,在叶片中,与对照相比,盐处理后吉8945有21个点明显上调,吉农大808中35个点上调表达,其中12个点在吉8945和吉农大808均上调表达且差异明显。选取其中6个点进行MALDI-TOF-MS/MS质谱分析,鉴定出两种蛋白质,分别为磷酸丙酮酸水合酶和核酮糖1,5-二磷酸羧化酶/加氧酶。Two hybrid rice strains,‘Ji 8945＇（salt-tolerant） and‘Jinongda 808＇（salt-sensitive）,were used in this experiment.Two-dimensional polyacrylamide gel electrophoresis and MALDI-TOF-MS/MS techniques were employed to isolate and characterize salt stress responsive proteins of hybrid rice seedlings in early period of 10-day old under normal and salt stress（100mM NaCl） conditions.The results showed that 21 or 35 up-regulated spots were significantly found in‘Ji 8945＇and‘Jinongda 808＇leaves compared with the control.Six of them were selected and identified by MALDI-TOF-MS/MS.Only two proteins were identified,i.e.,ribulose-1,5-bisphosphate carboxylase/oxygenase and enolase.

关 键 词：水稻 盐胁迫 蛋白质 双向电泳 MALDI-TOF-MS 

分 类 号：S511[农业科学—作物学]