林下参根区土壤微生物群落结构的研究  

Studied on Soil Microbial Community Structure about Wild Ginseng under Forest

作　　者：张亚玉[1,2] 孙海[2] 宋晓霞[2] 汪景宽[1] 

机构地区：[1]沈阳农业大学土地与环境学院,沈阳110161 [2]中国农业科学院特产研究所,吉林吉林132109

出　　处：《水土保持研究》2011年第2期169-173,共5页Research of Soil and Water Conservation

基　　金：吉林省科技厅支撑计划项目"林下人参;西洋参生产关键技术研究与示范"(20080905)

摘　　要：为了解林下参的生长对土壤微生物群落结构的影响,采用磷脂脂肪酸(PLFA)法测定了5,10,15,20,25年生林下参根区土壤的微生物量的活性部分及群落结构的变化情况。结果表明:林下参根区土壤微生物群落均以i15:0,a15:0,18:1ω9t,16:0,16:1ω9c,18:1ω9c,br17:0,18:2ω6,i16:0,16:1ω9t的微生物为优势种群;林下参生长根区土壤微生物总量、细菌生物量、真菌生物量及放线菌生物量与对照相比均有提高,且15a以后提高的幅度更加明显;在菌群结构变化上,与对照相比20a及25a放线菌占有总生物量的比率有所提高,真菌占有比率有所下降。15年后的林下参土壤微生物结构朝着有利于人参生长的微环境方向发展,野山参在深山密林中健康生长几十年而不死亡可能与其土壤微生物群落结构有直接关系。In order to study the effects of wild ginseng under forest on soil microbial community structures,active microbial mass and community structure were determined by phosphor lipid fatty acid（PLFA） in wild ginseng under forest with 5,10,15,20 and 25 years old different root zone soil.The results showed the microorganisms containing the PLFA of i15：0,a15：0,18：1ω9t,16：0,16：1ω9c,18：1ω9c,br17：0,18：2ω6 i16：0,and 16：1ω9t were dominance in microbial community in different years old wild ginseng under forest root zone soil;bacterial biomass,fungal biomass and actinomycetes biomass enhanced significantly;biomass more enhanced after 15 years old.In the community structure actinomycetes ratio of biomass increase and fungi ratio of biomass declined compared with contrasts in 20 years and 25 years.The structure of microorganisms is developing to good microenvironment for growing after 15 years.

关 键 词：林下参 土壤微生物群落结构 磷脂脂肪酸 

分 类 号：S714.3[农业科学—林学]