饥饿对褐菖鲉消化道指数及消化酶活力的影响  被引量:15

Effects of Starvation on Digestive Enzyme Activity and Digestive Tract Index in Dustry Stingfish Sebastiscus marmoratus

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作  者:江丽华[1] 朱爱意[1] 苑淑宾[1] 

机构地区:[1]浙江海洋学院浙江省海洋养殖装备与工程重点实验室,浙江舟山316000

出  处:《水产科学》2011年第4期187-191,共5页Fisheries Science

基  金:国家海洋局海洋公益性行业科研专项(201005013)

摘  要:在水温(18±0.5)℃、盐度27.0±0.5条件下,测定了褐菖鲉不同饥饿时间(0、3、7、14、21 d)的比肝质量、比胃质量、比幽门质量、比肠长及各消化组织消化酶活力。试验结果表明,在饥饿过程中,褐菖鲉消化道组织萎缩,比肝质量、比胃质量、比幽门质量和比肠长呈显著下降趋势(P<0.01),其中比肝质量下降速率最大,饥饿21 d的比肝质量比对照组下降85.033%。饥饿期间,蛋白酶活力总体呈先升后降的趋势,差异显著(P<0.05)。淀粉酶活力的变化趋势亦为先升后降,饥饿1 d,前、中、后肠、幽门的淀粉酶活力呈不同程度上升,之后,除胃和幽门外,随着饥饿时间的延长,皆呈下降趋势,与对照组相比,差异显著(P<0.05);脂肪酶活力总体呈缓慢下降趋势,差异不显著(P>0.05)。The effects of starvation(0,3,7,14,and 21 d)on the liver weight/body weight,stomach weight/body weight,pylorus weight/body weight,intestine length/body length and the activities of protease,amylase and lipase were studied in dustry stingfish Sebastiscus marmoratus at temperature of 18±0.5 ℃ and salinity of 27.0±0.5 in indoor conditions.It was found that the digestive organs all atrophied,liver weight/body weight,stomach weight/body weight,pylorus weight/body weight and intestine length/body length were all decreased during the whole starvation period,and liver weight/body weight decreased very significantly(P0.01),compared with the control group,the liver weight/body weight in 21 d starvation group being less than 85.033%.During the starvation period,the activities of protease were found to be increased firstly and then decreased,with significant difference(P0.05).The activities of amylase were also found to be increased firstly and then decreased.Activities of amylase in foregut,midgut,hindgut and pylorus more or less increased in 1 d starvation,and then decreased as starvation proceeded,significant different from the control group(P0.05).There was no significant difference in the activity of lipase in the starved fish though the lipase activity in the treatment groups tended to decrease(P0.05).

关 键 词:褐菖鲉 饥饿 消化道指数 消化酶活 

分 类 号:S917[农业科学—水产科学]

 

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