不同浓度的Cd^(2+)、氮及其交互作用对小球藻和微绿球藻生长及叶绿素荧光特性的影响  

The Interaction Effects of Cd^(2+) and N Concentrations on Growth and Chlorophyll Fluorescence Characteristics in Algae Chlorella sp.and Nannochloris oculata

作　　者：王帅[1,2] 梁英[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]国家海洋局第一海洋研究所海洋生物活性物质重点实验室,山东青岛266061

出　　处：《水产科学》2011年第4期210-214,共5页Fisheries Science

基　　金："十一五"国家科技支撑计划项目(2006BAD09A03);中国海洋大学海水养殖教育部重点实验室开放课题(200603)

摘　　要：研究了不同的Cd2+浓度与氮浓度交互作用对小球藻和微绿球藻生长及叶绿素荧光特性的影响。结果表明,Cd2+浓度、氮浓度、胁迫时间及三者的交互作用对小球藻和微绿球藻的生长及光合作用均具有显著影响(P<0.05),其中Cd2+浓度对2株微藻的Fv/Fm(光系统Ⅱ的最大光化学量子产量)和Yield(光系统Ⅱ的实际光能转化效率)影响最显著,氮浓度对2株微藻的NPQ(非光化学淬灭)和微绿球藻的qP(光化学淬灭)影响最显著。相关性分析结果表明,2株微藻的细胞密度、叶绿素相对含量及部分荧光参数(Fv/Fm和Yield)均与Cd2+浓度呈显著的负相关关系。在低氮(55μmol/L)条件下,小球藻和微绿球藻的荧光参数Fv/Fm、Yield和NPQ均有明显下降,细胞密度和叶绿素相对含量也均有不同程度的降低。小球藻的Fv/Fm和Yield在高氮(7040μmol/L)和低浓度Cd2+条件下,下降幅度小,表现为拮抗作用。试验还发现,在低氮和高氮条件下,高浓度Cd2+对小球藻的胁迫作用加强,表现为协同作用。微绿球藻在高氮条件下,其处理组间荧光参数差别不明显,表现为拮抗作用。The interaction effects of different Cd2＋ and N concentrations on growth and chlorophyll fluorescence parameters in green algae Chlorella sp.and Nannochloris oculata were investigated.By three-factor analysis of variance,Cd2＋ concentrations,N concentrations,stress time and their interaction were all showed to have significant effects on the chlorophyll fluorescence parameters,relative chlorophyll content and cell density of the algae（P0.05）,especially Cd2＋ concentrations having the very significant effect on the parameters Fv/Fm（maximal photochemical efficiency of PSII）and yield（actual photochemical efficiency of PSII）in the algae while N concentrations showing the very significant effect on both NPQ（non-photochemical quenching）in the algae and QP（photochemical quenching）in N.oculata.For both algae,there were significant negative correlations between the cell density,relative chlorophyll content and some fluorescence parameters（Fv/Fm and Yield）with Cd2＋ concentrations.The parameters Fv/Fm,yield and NPQ,as well as the cell density and relative chlorophyll content were decreased in the algae exposed to low N concentration（55 μmol/L）.Under high N concentration（7040 μmol/L）and low Cd2＋ concentration（20 μmol/L）,however,the parameters Fv/Fm and yield in Chlorella sp.reached the maximum,indicating that high N concentration led to reduce the Cd2＋toxicity in Chlorella sp.The effect of high Cd2＋ concentration was enhanced at low and high N concentrations in Chlorella sp.,which showed synergy.For N.oculata,there was no significant difference in fluorescence parameters,and antagonism among the Cd2＋treatment groups under high-N condition exposure.

关 键 词：小球藻 微绿球藻 Cd2+浓度 氮浓度 叶绿素荧光 

分 类 号：Q945.78[生物学—植物学]