成都地区黏细菌物种资源的分离和区域分布  

Isolation and Distribution of Species Resource of Myxobacteria in Chengdu Region

作　　者：刘冰花[1] 何柳兴[1] 

机构地区：[1]成都大学医护学院,成都610015

出　　处：《新疆农业大学学报》2011年第2期146-151,共6页Journal of Xinjiang Agricultural University

基　　金：四川省教育厅资助科研项目(08zb092);成都大学校基金项目(2009XJZ162010XJZ32)

摘　　要：利用兔粪诱导法、VY/4多菌灵琼脂培养基诱导法和无机盐琼脂培养基诱导法从来自成都各区县的水样、土样、兔粪、羊粪、朽木、风化岩石共35个样品中分离纯化黏细菌,经过生理、生化和形态学鉴定,共得到42株黏细菌。这42株黏细菌分属于黏球黏细菌属(Myxococcus)、粒状黏细菌属(Chondromyces)、囊球黏细菌属(Angiococcus)、原囊黏细菌属(Archangium)、蜂窝黏细菌属(Melittangium)、堆囊黏细菌属(Sorangium)和标桩黏细菌属(Stigmatella)7个属。按样品的采集地域、样品种类和生态环境进行了种群分布及多样性的统计分析,发现成都地区黏细菌的数量和种属均较多,特别是黄龙溪古镇河水一个样品就得到5个属的7株黏细菌。同时也发现旅游景区样品中黏细菌出菌率最高,其次是森林样品,草地样品和果树林样品出菌率最低。并且旅游景区黏细菌的得菌率和种属多样性明显高于其他环境。The myxobacteria are screened from 35 samples included water,mud,rabbit dung,goat dung,rotten wood,weathered rock,from different prefectures and counties of Chengdu by rabbit dung pellets inducing method,VY/4 carbendazim agar culture inducing method and mineral salt agar culture inducing method.Forty two myxobacteria strains are isolated from 7 genera,which belong to Myxococcus,Chondromyces,Angiococcus,Archangium,Melittangium,Sorangium and Stigmatella,and are detected by their physiological,biological and morphological characteristics.The statistical analysis of population distribution and diversity are done by comparing the collection sites of samples,the variety of samples and the ecological environment of collection sites,it is found that there are plentful quantity and genera of myxobacteria in Chengdu region.Especially,there are 7 myxobacteria strains in 5 genera from the river water samples of Huanglong ancient town.The study also shows that ratio of purification in the sample from tourist landscape area are the highest among the collection sites,and followed by the samples of forest,grassland and fruit gardens.The ratio of purification of myxobacteria in the tourist landscape area and resource diversity are higher than that of other invironment.

关 键 词：黏细菌 分离 得菌率 种群分布 资源多样性 成都 

分 类 号：Q938.1[生物学—微生物学]