反相高效液相色谱法测定盐酸左旋多巴正己酯的含量  

Determination of L-Dopa Hexyl Ester Hydrochloride by RP-HPLC

作　　者：吕聪[1] 蒋伟哲[1] 黄兴振[1] 巫玲玲[1] 施晓霞[1] 许崇摇[1] 邱卓[1] 

机构地区：[1]广西医科大学,广西南宁530021

出　　处：《中国药业》2011年第8期33-34,共2页China Pharmaceuticals

基　　金：广西自然科学基金项目;项目编号:桂科自0832014Z;广西研究生教育创新计划资助项目;项目编号:2009105981007M177

摘　　要：目的建立测定盐酸左旋多巴正己酯含量的高效液相色谱法。方法采用Ultimate C18色谱柱(250 mm×4.6 mm,5μm),以0.05 mol/L磷酸二氢钾(磷酸调pH至3.5)-乙腈(65∶35,V:V)为流动相,检测波长为280 nm,流速为1.0 mL/min,柱温为25℃。结果盐酸左旋多巴正己酯进样量在0.360～7.200μg范围内与峰面积线性关系良好,回归方程为Y=10 364 X+11 444,r=0.999 9(n=6);平均加样回收率为99.33%,RSD=1.87%(n=6)。结论所建立的方法操作简便,准确度、灵敏度高,可用于盐酸左旋多巴正己酯的含量测定。Objective To establish a HPLC method for the determination of L-dopa hexyl ester hydrochloride.Methods The separation was performed on the Ultimate C18 column（250 mm×4.6 mm,5 μm）.The mobile phase consisted of the mixture of 0.05 mol/L potassium dihydrogen phosphate（adjusting phosphoric acid to pH=3.5）-acetonitrile（65 ∶35,V ∶V）,with the detection wavelength at 280 nm.The flow rate was 1.0 mL/min and the column temperature was 25 ℃.Results The calibration curve was linear within the sample size range of 0.360-7.200 μg,the regression equation was Y=10 364X＋11 444,r=0.999 9（n=6）.The average recovery rate was 99.33%,RSD=1.87%（n=6）.Conclusion The method is simple with high accuracy and sensitivity,which can be used for the content determination of L-dopa hexyl ester hydrochloride.

关 键 词：反相高效液相色谱法 盐酸左旋多巴正己酯 含量测定 

分 类 号：R927.2[医药卫生—药学] R971.5