巨大芽孢杆菌Bacillus megaterium NCIB 8508蔗糖磷酸化酶生物催化合成α-熊果苷的研究  

Biocatalytic Synthesis of α-Arbutin by Bacillus Megaterium NCIB 8508 Sucrose Phosphorylase

作　　者：李群良[1] 张欣英[1] 杨克迪[1] 姚评佳[2] 魏远安[2] 

机构地区：[1]广西大学化学化工学院,广西南宁530004 [2]广西大学广西亚热带生物资源保护利用重点实验室,广西南宁530004

出　　处：《化学与生物工程》2011年第4期46-48,69,共4页Chemistry & Bioengineering

基　　金：广西应用基础研究专项项目(桂科基0991004)

摘　　要：利用巨大芽孢杆菌Bacillus megateriumNCIB 8508蔗糖磷酸化酶生物催化合成α-熊果苷。考察了缓冲溶液pH值、缓冲溶液浓度、对苯二酚浓度、反应物摩尔比及反应时间等因素对反应的影响。结果表明,以湿菌体50 mg.mL-1破碎细胞粗酶液催化合成α-熊果苷,在pH值为6.5的30 mmol.L-1磷酸盐缓冲溶液中,温度为30℃、摇床转速为180 r.min-1、对苯二酚浓度为5 mmol.L-1、对苯二酚与蔗糖摩尔比为1∶5、反应时间为24 h的优化条件下,α-熊果苷含量达0.35 mg.mL-1、对苯二酚选择性为4.0%、对苯二酚转化率为74%。The biocatalytic synthesis of α-arbutin by Bacillus megaterium NCIB 8508 sucrose phosphorylase was studied in this paper.The effects of pH value of buffer solution,buffer solution concentration,hydroquinone concentration,reactant molar ratio,and reaction time were investigated.The results showed that with crude enzyme from 50 mg·mL-1 wet cells,α-arbutin was synthesized under the optimized reaction conditions as follows：buffer solution pH value of 6.5,buffer solution concentration of 30 mmol·L-1,temperature of 30℃,shaking speed of 180 r·min-1,hydroquinone concentration of 5 mmol·L-1,molar ratio of hydroquinone to sucrose of 1∶5,and reaction time of 24 h,the α-arbutin content was 0.35 mg·mL-1,the selectivity of hydroquinone was 4.0%,and the conversion rate of hydroquinone was 74%.

关 键 词：巨大芽孢杆菌 蔗糖磷酸化酶 α-熊果苷 生物催化 选择性 

分 类 号：O621.3[理学—有机化学] Q55[理学—化学]