纤溶酶原激活剂及其抑制因子1在大鼠脊髓急性损伤时的表达  

Expression of Tissue-type Plasminogen Activator and Its Inhibitor-1 in Acute Rat Spinal Cord Injuries

作　　者：王德春[1,2] 陈中伟[1,2] 陈峥嵘[1,2] 张乃娴[1,2] 宋后燕[1,2] 

机构地区：[1]上海医科大学附属中山医院骨科 [2]上海医科大学分子遗传研究室

出　　处：《中华创伤杂志》1999年第4期266-268,共3页Chinese Journal of Trauma

摘　　要：目的观察大鼠脊髓急性损伤时组织型纤溶酶原激活剂（ｔ－ＰＡ）和纤溶酶原激活剂抑制因子１（ＰＡＩ－１）表达的变化，了解ｔ－ＰＡ、ＰＡＩ－１在脊髓急性损伤后所起的作用。方法３５只Ｗｉｓｔａｒ大鼠，用改良Ａｌｅｎ法制成脊髓中度急性损伤模型，伤后２，６，２４，７２，１６８，２４０小时取材分别进行组织学、免疫组化、图像分析。结果脊髓中度损伤后，损伤部位脊髓经过了出血、血栓形成、液化、坏死等病理变化。脊髓损伤后３天ｔ－ＰＡ表达达到高峰，以后逐渐下降，而ＰＡＩ－１伤后３天有少量表达，以后逐渐增多。结论ｔ－ＰＡ、ＰＡＩ－１参与脊髓急性损伤过程，ＰＡＩ－１对保护残存的运动神经元及胶质细胞具有一定作用。Objective To observe the changes of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) expression in acute rat spinal cord injuries (SCI), to learn about the effect of t-PA and PAI-1 on the process of acute spinal cord injury. Methods Using thirty-five Wistar rats, the moderately acute spinal cord injury models were made with modified Allien's methods. Histology examination, immunohistochemistry and map analyses were performed after 2, 6, 24, 72, 168, 240 hours of injury. Results After moderately acute spinal cord injury, pathology changes such as bleeding, thrombosis, liquescence, necrosis, etc occurred in the local area of injured spinal cord. Immunohistochemistry and map analysis demonstrated that the expression of t-PA reached the maximum in 3 days of injury, and subsequently reduced; but only a small amount of PAI-1 was expressed in 3 days of SCI, and then increased gradually. Conclusions t-PA and PAI-1 participate in the process of acute spinal cord injury. PAI-1 has some effects on protecting the remnant motor neurons and gliocytes.

关 键 词：脊髓损伤 组织病理 抑制因子1 T-PA 

分 类 号：R651.2[医药卫生—外科学]