三七总皂甙对颅脑损伤后Ca^(2+)、CaM含量影响的实验研究  

Effect of Panax Notoginseng Saponins on the Change of Ca 2+ , CaM Contents Following Brain Injury

作　　者：韩金安[1,2] 匡永勤[1,2] 周虎田[1,2] 孙增会[1,2] 李开慧[1,2] 胡威夷[1,2] 

机构地区：[1]襄樊解放军第四七七医院神经外科 [2]成都军区总医院神经外科

出　　处：《中华创伤杂志》1999年第4期278-280,共3页Chinese Journal of Trauma

摘　　要：目的探讨三七总皂甙（ＰＮＳ）对颅脑损伤后脑组织中Ｃａ２＋、ＣａＭ含量的影响及其对颅脑损伤后的治疗效应。方法干湿法测定脑组织含水量，放免法、原子吸收分光光度法测定血及脑组织中Ｃａ２＋、ＣａＭ含量；分析ＰＮＳ对上述指标的影响。结果ＰＮＳ能显著降低颅脑损伤后血与脑组织中Ｃａ２＋、ＣａＭ含量，减少脑组织含水量及伊文思蓝染范围。结论ＰＮＳ能阻滞脑损伤后神经细胞内钙超载，阻断Ｃａ２＋－ＣａＭ复合物形成，减轻脑水肿和血脑屏障通透性，对颅脑损伤具有一定的脑保护作用。Objective To study the effects of the panax notoginseng saponins (PNS) on the changes of Ca 2+ , CaM content and the brain protective effects after brain injury. Methods A model of local cerebral injury on rabbits was set up by a drop of 100 g weight at 20 cm height hitting on the left epidura, then 100 mg/kg PNS was intravenously injected at 30 min and 12 h after brain injury. The contents of Ca 2+ , CaM in blood and brain tissue were measured by atonic absorption spectrometry and radioimmunoassay. Results PNS significant reduced Ca 2+ , CaM contents both in blood and cerebral tissue ( P <0.01). Conclusions PNS can reduce Ca 2+ , CaM contents in brain tissue, block Ca 2+ overload in neurons, consequently inhibit the production of Ca 2+ -CaM compound that causes neurons damage. PNS has a protective effect on brain injury.

关 键 词：颅脑损伤 钙 钙调蛋白 三七总皂甙 实验研究 

分 类 号：R651.15[医药卫生—外科学] R285.5[医药卫生—临床医学]