离子色谱法测定中药材中总二氧化硫残留  

Determination of Total Sulfur Dioxide in Traditional Chinese Medicinal Materials by Ion Chromatography

作　　者：孙磊[1] 岳志华[1] 陈佳[1] 金红宇[1] 马双成[1] 林瑞超[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中国药事》2011年第4期336-338,共3页Chinese Pharmaceutical Affairs

基　　金：国家十一五科技支撑项目:矿物药中金属含量检测技术及中药中外源性有害残留物检测技术研究(编号2008BA155B02);中药中有害残留物检测技术标准平台(编号2009ZX09308-006)

摘　　要：目的建立并优化离子色谱法测定中药材中总二氧化硫残留。方法样品采用加酸水蒸汽蒸馏法提取,用3%过氧化氢溶液吸收二氧化硫并氧化为硫酸根,以20mmol.L-1KOH溶液为淋洗液经IonPacAS-11-HC离子色谱柱分离,电导检测器检测。结果硫酸根在0.002~2μg范围内线性良好（r=1）,三水平回收率为83.3%~100.0%,RSD为0.5%~4.7%,方法检测限为0.05mg.kg-1,定量限为0.13mg.kg-1。结论本法快速、简便、专属、灵敏,适用于中药材中总二氧化硫残留的检测。Objective To establish and optimize an ion chromatography method for determination of total sulfur dioxide residues in traditional Chinese medicinal materials.Methods The samples were extracted by steam distillation with acid and absorbed with hydrogen peroxide solution.Test solution were separated by IonPac AS-11-HC column using 20mmol·L-1 potassium hydroxide solution as eluent and detected with conductivity detector.Results The linear range of sulfate radical was 0.002~2μg（r=1）.The average recoveries in three levels were 83.3%~100.0% with RSD between 0.5%~4.7%.The limit of detection was 0.05mg·kg-1,and the limit of quantitation was 0.13mg·kg-1.Conclusion The method is rapid,simple,specific,sensitive and suitable for determination of sulfur dioxide residues in traditional Chinese medicinal materials.

关 键 词：离子色谱法 中药材 二氧化硫 亚硫酸盐 

分 类 号：R917[医药卫生—药物分析学] R282[医药卫生—药学]