HPLC法测定冠心康颗粒中芍药苷的含量  

Determination of Paeoniflorin in Guanxinkang Granules by HPLC

作　　者：郑建锋 郑阿利 盖轲[3] 

机构地区：[1]甘肃省西峰制药有限责任公司,庆阳745000 [2]甘肃省庆阳市药品检验所 [3]甘肃省陇东学院化学化工学院

出　　处：《中国药事》2011年第4期377-378,381,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立以HPLC法测定冠心康颗粒中芍药苷含量的方法。方法采用phenomenex luna C18色谱柱;流动相：甲醇-水（27∶73）;流速：0.8mL.min-1;检测波长：230nm;柱温：30℃。结果芍药苷在6.029~60.29μg.mL-1范围内线性关系良好,r=0.9999,平均回收率为99.53%,RSD为0.79%。结论该方法简便、可行,重复性好,可作为冠心康颗粒的质量控制方法。Objective To establish a HPLC method to determine the content of paeoniflorin in Guanxinkang Granules.Methods Phenomenex luna C18 column was used as chromatographic column.The mobile phase was methanol-water（27∶73）.The flow rate was 0.8mL·min-1,and detection wavelength was at 230nm.The column temperature was 30℃.Results The good linear range of paeoniflorin was 6.029~60.29μg·mL-1,r=0.9999.The average recovery was 99.53%,RSD=0.79%.Conclusion The method is simple,feasible and reproducible.It can be used for the quality control of Guanxinkang Granules.

关 键 词：芍药苷 冠心康颗粒 HPLC 

分 类 号：R284[医药卫生—中药学] R927.2[医药卫生—中医学]