HPLC法测定降脂灵胶囊中紫丁香苷及葛根素的含量  

Determination of Syringin and Puerarin in Jiangzhiling Capsules by HPLC

作　　者：张禹[1] 吴高芬 

机构地区：[1]湖南省岳阳市中医院,岳阳414000 [2]岳阳市药品检验所

出　　处：《中国药事》2011年第4期382-383,388,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立HPLC法测定降脂灵胶囊中紫丁香苷及葛根素的含量。方法采用Phenomenex luna-C18色谱柱;流动相：乙腈-水（1∶10）为流动相,检测波长：264nm;流速：1.0mL.min-1;柱温30℃。结果紫丁香苷和葛根素分别在0.067~1.686μg、0.020~0.508μg范围内呈良好线性关系,r分别为0.9999、1,其平均回收率分别为96.4%、98.0%,RSD分别为1.0%、0.5%。结论本方法操作简便、结果准确,专属性强。Objective To establish a HPLC method for determination of syringin and puerarin in Jiangzhiling Capsules.Methods Phenomenex Luna C18 column was used.The mobile phase was consisted of acetonitrile-water（1∶10）,and the detection wavelength was 264nm.The flow rate was 1.0mL ·min-1 and the temperature of column was 30℃.Results The standard curves of syringin and puerarin were linear in the range of 0.067～1.686μg（r=0.9999）,0.020～0.508μg（r=1）,respectively.The average recoveries were 96.4% and 98.0%,respectively.Conclusion The method is simple,accurate and specific.

关 键 词：高效液相色谱法 降脂灵胶囊 紫丁香苷 葛根素 

分 类 号：R284[医药卫生—中药学] R927.2[医药卫生—中医学]