过敏性紫癜P-选择素基因-2123位点多态性检测与临床分析  

P-selectin gene-2123 polymorphism in children with Henoch-Schnlein purpura

作　　者：李晶[1] 王一冰[1] 刘华林[1] 蒋玉红[2] 刘薇[2] 王亚秋[2] 

机构地区：[1]青岛市妇女儿童医疗保健中心内二科,山东青岛266011 [2]青岛市妇女儿童医疗保健中心检验科,山东青岛266011

出　　处：《中国当代儿科杂志》2011年第4期278-281,共4页Chinese Journal of Contemporary Pediatrics

基　　金：青岛市科技计划项目(06-2-2-5-nsh)

摘　　要：目的研究P-选择素基因-2123位点多态性与儿童过敏性紫癜(HSP)发病的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法对86例HSP患儿(其中40例合并肾炎)和70例健康对照进行基因多态性检测。结果与对照组比较,HSP患儿P-选择素基因-2123位点GG基因型频率和G等位基因频率均明显增高(P<0.05)。合并肾炎患儿与未合并肾炎患儿比较,该位点各基因型频率及等位基因频率差异均无统计学意义(P>0.05)。结论 P-选择素基因-2123位点多态性可能与儿童HSP发病有关。Objective To investigate whether P-selectin gene-2123 polymorphism is associated with the pathogenesis of Henoch-Schnlein purpura（HSP） in children.Methods Polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP） is used to identify the distribution of allele and genotype frequencies of P-selectin gene promoter-2123 polymorphism in 86 children with HSP（including 40 cases of purpura nephritis） and 70 healthy controls.Results Compared with the healthy controls,the frequencies of GG genotype and G allele of P-selectin promoter-2123 in children with HSP increased significantly（P0.05）.There were no significant differences in P-selectin promoter-2123 genotype and allele frequencies between the patients with and without nephritis.Conclusions P-selectin gene promoter-2123 polymorphism appears to be associated with the pathogenesis of HSP in children.

关 键 词：过敏性紫癜 肾炎 P-选择素 基因多态性 儿童 

分 类 号：R554.6[医药卫生—血液循环系统疾病]