肿瘤坏死因子-α干预对热性惊厥患儿外周血单个核细胞ICAM-1和LFA-1表达的影响  

Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures

作　　者：孙丹[1] 刘智胜[1] 姚辉[2] 康世秀[2] 何彩英[1] 胡家胜[1] 吴革菲[1] 王芳琳[1] 

机构地区：[1]武汉市儿童医院神经内科,湖北武汉430016 [2]武汉市儿童医院中心实验室,湖北武汉430016

出　　处：《中国当代儿科杂志》2011年第4期285-287,共3页Chinese Journal of Contemporary Pediatrics

基　　金：武汉市科技局武汉青年科技晨光计划资助项目(编号20015005050)

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)对热性惊厥(FS)患儿外周血单个核细胞(PBMC)细胞间黏附分子-1(ICAM-1)及配体淋巴细胞功能相关抗原-1(LFA-1)表达的影响。方法 16例FS患儿随机分为FS对照组和TNF-α干预组。TNF-α干预组采用1.0 ng/mL的TNF-α进行干预。对照组16例,为年龄和性别相匹配的同期体检健康儿童。进行外周血PBMC培养。流式细胞仪检测PBMC表面ICAM-1和LFA-1的表达。结果 FS对照组在体外培养的PBMC表面ICAM-1和LFA-1表达水平分别为(20±9)%和(43±16)%,明显高于正常对照组[(14±7)%,(30±16)%](P<0.05);TNF-α干预组的PBMC表面ICAM-1表达水平[(27±11)%]明显高于FS对照组(P<0.05),LFA-1表达[(52±21)%]较FS对照组有增高趋势,但差异无统计学意义。结论炎症介质TNF-α刺激可使FS患儿的LFA-1和ICAM-1表达上调。Objective To study the effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells（PBMC） of children with febrile seizures（FS）.Methods Sixteen children with FS and 16 age-and gender-matched healthy children were enrolled.The samples of PBMC from FS children were randomized into two groups with or without TNF-α treatment（TNF-α concentration 1.0 ng/mL）.PBMC were purified and cultured with a conventional method in vitro.The expression of ICAM-1 and LFA-1 in PBMC was determined by flow cytometry（FCM）.Results ICAM-1 [（20±9）% vs（14±7）%）] and LFA-1 [（43±16）% vs（30±16）%] expression in PBMC in the untreated FS group was significantly higher than that in the normal control group（P0.05）.Compared with the untreated FS group,the treatment with TNF-α remarkably increased the ICAM-1 expression [（27±11）%]（P0.05）.PBMC LFA-1 expression [（52±21）%] in the TNF-α-treated group was higher than that in the untreated FS group,although there were no statistical differences between the two groups.Conclusions TNF-α treatment may increase LFA-1 and ICAM-1 expression in PBMC of children with FS.

关 键 词：肿瘤坏死因子-Α 热性惊厥 ICAM-1 LFA-1 外周血单个核细胞 儿童 

分 类 号：R720.597[医药卫生—急诊医学]