α-硫辛酸对糖尿病大鼠肾脏聚ADP-核糖多聚酶表达的影响  

Effect of α-lipoic acid on the expression of poly-ADP-ribose polymerase in the kidney of diabetic rats

作　　者：陈艳[1] 吴晨光[1] 王丽[1] 徐志刚[1] 陈宇宁[1] 李文[1] 

机构地区：[1]江苏大学附属人民医院内分泌病科,镇江市212002

出　　处：《江苏医药》2011年第6期624-626,共3页Jiangsu Medical Journal

基　　金：江苏省卫生厅面上项目(H200830);镇江市医学重点人才基金[镇卫字(2005)237号]

摘　　要：目的探讨α-硫辛酸(α-LA)对糖尿病大鼠肾脏聚ADP-核糖多聚酶(PARP)表达的影响。方法 30只SD大鼠随机均分为链脲佐菌素(STZ)诱导的糖尿病组(A组)、α-LA干预的糖尿病组(B组)及正常对照组(C组)。8周后测各组大鼠肾脏超氧化物歧化酶(SOD)、丙二醛(MDA)含量,TUNEL法检测肾脏细胞凋亡,免疫组化法观察PARP在肾脏中的表达,Western blot法检测肾脏半胱氨酸蛋白酶3(Caspase-3)的表达。结果与C组比,A组大鼠肾脏SOD活性下降,MDA含量、肾脏细胞凋亡数、PARP和Caspase-3的表达均显著增多(P<0.05),而B组上述指标均较A组明显改善(P<0.05)。结论α-LA对糖尿病大鼠肾脏保护作用可能与抑制氧化应激、下调PARP表达而对抗细胞凋亡有关。Objective To investigate the effect of α-lipoic acid（α-LA） on the expression of poly-ADP-ribose polymerase（PARP） in the kidney of diabetic rats.Methods Thirty SD rats were randomly and equally divided into three groups of A[streptozotocin（STZ）-induced diabetes mellitus（DM） model],B（DM treated with α-LA） and C（normal control）.After 8 weeks,superoxide dismutase（SOD） and malondialdehyde（MDA） were detected in the kidney.The cell apoptosis in glomerular and tubular was counted by TUNEL immunofluorescence assay.The expressions of PARP and Caspase-3 were detected by immunohistochemistry and Western blot,respectively.Results Compared with group C,the activity of SOD was decreased,the content of MDA,the number of cell apoptosis and the expressions of PARP and Caspase-3 were all significantly increased in group A（P0.05）,which were much more improved in group B than those in group A（P0.05）.Conclusion α-LA protects the kidney of DM rats probably by reducing cell apoptosis via inhibiting oxidative stress and decreasing the expression of PARP.

关 键 词：Α-硫辛酸 糖尿病肾病 聚ADP-核糖多聚酶 氧化应激 细胞凋亡 

分 类 号：R587[医药卫生—内分泌]