马兜铃酸Ⅰ致小鼠肾脏损伤的研究  

The Study of Mouse Kidney Injury by Aristolochic Acid Ⅰ

作　　者：董晓凯[1] 张中文[1] 彭晓兰[1] 廖芳芳[1] 高磊[1] 吴国娟[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《畜牧兽医学报》2011年第4期572-577,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30771625;30972215);北京市自然科学基金项目(6092004;5102014);北京市属高校人才强教计划资助项目(PHR2009-07135)

摘　　要：本研究旨在观察马兜铃酸Ⅰ对小鼠肾脏超微结构及其相关生理指标的影响,初步阐明马兜铃酸Ⅰ对肾脏的损伤作用,为探讨发病机理提供理论依据。将20g左右的雄性昆明鼠随机分成4组,每日分别用低、中、高3个浓度的马兜铃酸Ⅰ溶液灌胃,对照组用等体积饮用水替代。第30天时观察各组的肾脏组织病理学变化,并检测血液和尿液中相关生理指标。随着给药剂量的增加,小鼠体质量呈加速下降趋势,初期尿量增多,后期尿量减少甚至出现无尿、肾性糖尿,而血清中TGF-β1的含量逐渐上升,小鼠红细胞、血红蛋白、单核细胞减少。与对照组相比,在皮质到皮髓交界处,试验组表现为近曲小管上皮细胞损伤,纤维化随着剂量的加大由皮质向髓质逐渐深入。电镜下观察可见细胞器损伤以及细胞核变异。马兜铃酸I对小鼠明显的剂量依赖性的损伤作用,主要导致肾小管间质病变,并改变其正常的超微结构。To provide theoretical basis for investigating pathologic mechanism,we observed the effects of aristolochic acidⅠ（AAⅠ）on kidney fine structures and physiological index of mice to preliminary interpret the damage of AA Ⅰon kidney.Male Kunming mice（20 g±0.2 g）were divided into four groups,randomly.The experimental groups were intragastricly administrated with low,moderate,and high concentration of AA Ⅰ solution,and the control group with equal-volume water.After 30 days,we examined pathological changes of kidney,and measured correlated physiological index in blood and urine.The results showed that with the increasing doses,mice body weights lowered obviously,urinary volumes increased in the initial stage,and then hypourocrinia,anuria,renal glycosuria occurred.While the TGF-β1 contents in serum went up gradually,and erythrocytes,hemoglobins,monocytes decreased.Compared with the control group,in cortex parts and the boundary between cortex and medulla parts,epithelial cells of proximal convoluted tubules damaged,and fibration penetrated deeply with the increasing doses from cortex to medulla parts.At the same time,we found that cell organs harmed and nuclei varied in electron microscope.The results showed that AAⅠ could damage kidney dose-dependently,lead mostly to pathological changes of renal tubule mesenchymal,and affect its normal fine structures.

关 键 词：马兜铃酸Ⅰ 肾脏 TGF-Β1 病理学变化 小鼠 

分 类 号：S852.3[农业科学—基础兽医学]