山羊支原体山羊肺炎亚种湖北株的鉴定及其外膜蛋白的免疫原性研究  

Identification of Mycoplasma capricolum subsp.capripneumoniae Hubei Strain in China and the Immunogenicity Study of Its Outer Membrane Proteins

作　　者：赵萍[1] 郭晗[1] 贺英[1] 高鹏程[1] 张念章[1] 逯忠新[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部草食动物疫病重点开放实验室农业部畜禽病毒学重点开放实验室,兰州730046

出　　处：《畜牧兽医学报》2011年第4期593-599,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：甘肃省科技支撑计划项目(1011NKCA054);甘肃省农业生物技术专项(GNSW-2010-09);国家科技基础性工作专项计划(2008FY210200)

摘　　要：从湖北某地山羊肺脏中分离支原体,经多次克隆纯化后进行生化试验、显微镜观察、PCR及酶切分析、基因特征鉴定以及用动物致病性试验对该菌株进行了鉴定,命名为GH2,进一步通过免疫印迹对GH2株外膜蛋白及GH2株全菌蛋白免疫原性进行分析,并用抗Y98血清和抗PG3血清进行比较,筛选GH2株特异性的外膜蛋白,结果表明GH2株为山羊支原体山羊肺炎亚种,存在于GH2外膜蛋白中的相对分子质量大约为60和49.7ku的蛋白可能是其主要的免疫原性蛋白,也是GH2株区别于丝状支原体山羊亚种标准株PG3和绵羊肺炎支原体标准株的主要特异性抗原。这一结果为山羊支原体山羊肺炎亚种的诊断和疫苗研制提供了良好的理论基础。A strain of Mycoplasma named GH2 was isolated successfully from lung tissues of sick goats in Hubei province.This isolate was purified several times and were identified by biochemistry test,microscope observation,PCR,REA,genome analysis and animal pathogenicity tests.The immunogenicity of outer membrane proteins and whole cell proteins of GH2 were analyzed by western blotting.The results were compared with antiserum of Mycoplasma mycoides subsp.capri and Mycoplasma ovipneumoniae.Result showed that the strain GH2 belongs to the Mycoplasma capricolum subsp.capripneumoniae（Mccp） and the proteins about 60 and 49.7 kD possess its capital immunogenicity and they were also the specific antigen of GH2 which distinguished with Mycoplasma mycoides subsp.capri and Mycoplasma ovipneumoniae.Result of this study lay good theoretical foundation for diagnosis and vaccine development of Mycoplasma capricolum subsp.capripneumoniae.

关 键 词：山羊支原体山羊肺炎亚种 鉴定 外膜蛋白 免疫原性 

分 类 号：S852.62[农业科学—基础兽医学]