胰岛素注射方式对2型糖尿病KK小鼠血脂的影响  

Effects of insulin treatment on blood lipid in type 2 diabetic KK mice

作　　者：张超[1,2] 邓珊珊[1,2] 徐蓉[1,2] 王欣然[1,2] 唐丽娜[1,2] 相莉[3] 孙洪范[1,2] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所 [2]天津市生物医学材料重点实验室,天津300192 [3]天津市人民医院内分泌科,天津300121

出　　处：《中国临床药理学与治疗学》2011年第2期130-134,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：天津市自然科学应用技术重点资助项目(08JCZDJC16000)

摘　　要：目的：观察胰岛素腹腔和皮下给药对2型糖尿病模型（KK小鼠）血脂的影响。方法：选择空腹血糖高于20 mmol/mL的KK小鼠作为2型糖尿病模型,分为腹腔给药组（腹腔给药组,n=9）和皮下给药组（皮下给药组,n=9）。C57BL小鼠作为正常对照（正常对照组,n=10）。2型糖尿病KK小鼠连续给予高糖高脂饮食4周,同时IP组每日一次注射胰岛素0.3 mL,皮下给药组每日一次注射胰岛素0.4 mL（速效胰岛素？精蛋白锌胰岛素=2？1）,以维持空腹血糖正常（6.0±1.5）mmol/L。结果：胰岛素治疗2~4周,腹腔胰岛素给药可使2型糖尿病KK小鼠血清TG、TC、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）的浓度达到正常水平。皮下胰岛素给药只能使TC达到正常水平。结论：与胰岛素皮下给药相比,腹腔胰岛素给药能够更有效地纠正2型糖尿病KK小鼠脂代谢异常。AIM： To study the effect of intraperitoneal and subcutaneous insulin administration on serum lipids in a type 2 diabetic animal model（KK mice）. METHODS： KK mice with fasting blood sugar level higher than 20 mmol/mL were set as type 2 diabetic model and divided into subcutaneous injection group（s.c.group,n=9） and introperitoneal injection group（i.p.group,n=9）.C57BL mice were set as normal control（C group,n=10）.The type 2 diabetic KK mice were given high glucose and fatty for 4 weeks.Meanwhile,insulin was administrated at doses of 0.4 mL/d for s.c.group and 0.3 mL/d for i.p.group to normalize fasting blood glucose concentration（6.0 mmol/L±1.5 mmol/L）.RESULTS：After the diabetic KK mice were treated with insulin for 2-4 weeks,the serum TG,TC,LDL-C and HDL-C were recovered to the normal scope by intraperitoneal injections of insulin,but TG,LDL-C and HDL-C were not recovered to the normal level by subcutaneous injections of insulin.CONCLUSION： This result indicates that compared with subcutaneous insulin administration,intraperitoneal insulin administration is more efficacious to correct the disorder of lipid metabolism.

关 键 词：糖尿病 胰岛素 脂代谢 

分 类 号：R965.2[医药卫生—药理学]