黄连、吴茱萸及其药对对大鼠结肠异变腺窝的影响  

Influence of huanglian,wuzhuyu and huanglian-wuzhuyu pair herbs on aberrant crypt foci(ACF) in rat colon

作　　者：周昕[1,2] 董立[3] 黄波[1] 吴大正[2] 

机构地区：[1]上海中医药大学附属龙华医院药剂科,上海200032 [2]上海中医药大学,上海201203 [3]山东省泰安市中心医院

出　　处：《中国临床药理学与治疗学》2011年第2期143-147,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(30171147)

摘　　要：目的:研究黄连、吴茱萸及其药对对大鼠结肠癌前病变(异常腺窝病灶,aberrant crypt foci,ACF)的影响。方法:皮下注射二甲肼(1,2-di m-ethylhydrazine,DMH)诱发大鼠结肠ACF形成,根据形态法观察亚甲蓝染色后ACF在结肠上皮的数量和分布变化。同时通过测定肝重指标评估药物对肝的损伤情况。结果:与模型组相比,黄连、吴茱萸及其药对在大剂量和中剂量时,都能明显抑制ACF形成(P<0.01);在小剂量时,黄连组亦能抑制ACF形成(P<0.05);黄连大、中剂量组和吴茱萸大剂量组的肝重指数有所增加(P<0.01),而药对组的肝重指数小于黄连组及吴茱萸组,且3给药组间有统计学差异(P<0.01)。结论:黄连、吴茱萸及其药对在一定程度上能抑制ACF形成,且配伍后能减轻对肝的损伤。AIM： To study the influence of huanglian,wuzhuyu and huanglian-wuzhuyu pair herb on aberrant crypt foci（ACF） in rat colon. METHODS： DMH（1,2-dimethylhydrazine） was injected subcutaneously to induce ACF in rat colon.According to morphologic criteria,the ACF＇s frequencies and position were observed after stained with methylene blue,and live dysfunction induced by medicines were evaluated by liver weight index.RESULTS：The numbers of ACF were significantly decreased by huanglian,wuzhuyu and huanglian-wuzhuyu pair herb（P0.01）;herb pair adverse function for liver was better than single herb.CONCLUSION： It is concluded that huanglian,wuzhuyu and huanglian-wuzhuyu pair herb may be potential chemoprevention agents against colorectal cancer;pair herb may have less adverse effect than single herb.

关 键 词：黄连 吴茱萸 黄连吴茱萸药对 异常腺窝病灶 

分 类 号：R965.2[医药卫生—药理学]