急性应激对大鼠行为学及蓝斑TH、DBH基因表达的影响  

Effects of acute stress on emotional behaviors and the expression of tyrosine hydroxylas and dopamine-β-hydroxylase in locus coeruleus of rats

作　　者：陶杨[1] 丁秀芳[1] 陈育尧[1] 孙学刚[1] 覃桂强[1] 张艳平[1] 黄杰春[1] 吕志平[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515

出　　处：《热带医学杂志》2011年第3期274-277,共4页Journal of Tropical Medicine

摘　　要：目的观察急性应激状态下大鼠行为学的改变及不同时间点蓝斑内酪氨酸羟化酶(TH)和多巴胺-β-羟化酶(DBH)的基因表达。方法将24只健康雄性Wistar大鼠随机分为空白对照组和模型组,模型组又分为造模后1、3、6h组,每组6只。运用束缚和游泳的方法造模,观察其行为学的改变;并分别在造模后相应时间点取出蓝斑,用RT-PCR法检测蓝斑TH、DBH的表达。结果模型组造模前后比较,1、6h组的穿越格数和直立次数明显下降(P<0.05),3h组的穿越格数、直立次数和理毛次数均明显降低(P<0.05,P<0.05,P<0.01);与对照组大鼠相比,造模后1h组的穿越格数减少(P<0.05),1、3h组的直立次数明显降低(P<0.01,P<0.05),1、3、6h组的理毛次数降低明显且差异均有统计学意义(均P<0.01)。模型组蓝斑TH和DBH表达水平较对照组升高,其中3h组表达最高(P<0.05),1、6h组差异均无统计学意义(P>0.05),TH和DBH两者的表达趋势相似。结论急性应激使大鼠的行为能力降低,蓝斑去甲肾上腺素能神经元中TH、DBH表达增高,使去甲肾上腺素合成增多,而去甲肾上腺素可以显著影响大鼠的情绪行为,在应激过程中起重要作用;TH、DBH表达的增高可能参与了急性应激所致的行为异常。Objective To observe the emotional behaviors and stress-induced expression of tyrosine hydroxylas and dopamine-β-hydroxylase in the locus coeruleus.Methods Twenty-four healthy male rats were randomly divided into control group and experimental groups.Rats of the experimental groups received acute stress（i.e.restriction of mobility and swimming） and then the emotional behaviors were examined.Locus coeruleus were collected from the decapitated heads at 1 h,3 h,and 6 h after the stress application.The levels of mRNA of TH and DBH in the locus coeruleus were determined by RT-PCR.Results After the acute stress,the traversing grid decreases in group 1 h,3 h and 6 h（P〈0.05）,erect time also reduces in three model groups（P〈0.05,P〈0.01）,and grooming time only decreases in group 3 h（P〈0.05）.Compared with control group,the traversing grid reduces in group 1 h（P〈0.05）,erect time decreases in group 1 h and 3 h（P〈0.01,P〈0.05）,and grooming time with all model groups have statistical significance（P〈0.01）.The levels of mRNA of TH and DBH in the locus coeruleus of the treatment groups were higher than the control group,and the pression of them reached the peak 3 h（P〈0.05）,both group 1 h and 6 h not have statistical significance（P〉0.05）.The mRNA pression of TH and DBH have similar trend.Conclusion After the acute stress,the behavior capability of Rats have obvious reduction,and the increase of TH and DBH expression in LC can synthesize more norepinephrine.Norepinephrine can affect emotional behaviors of rats and plays an important role in the acute stress.TH and DBH may play a role in the abnormality of emotional behaviors of rats induced by acute stress.

关 键 词：急性应激 行为学 蓝斑 酪氨酸羟化酶 多巴胺-Β-羟化酶 

分 类 号：Q55[生物学—生物化学]