吗啡持续刺激对小鼠树突状细胞成熟的影响  

Effects of sustained morphine stimulation on the maturation of mouse bone marrow-derived dendritic cell

作　　者：路海[1] 胡景霞[1] 白波[1] 

机构地区：[1]济宁医学院神经生物学研究所,济宁272067

出　　处：《现代免疫学》2011年第2期126-129,共4页Current Immunology

基　　金：国家自然科学基金项目(30971081;30870932;81070961);山东省自然基金资助项目(ZR2009DZ004)

摘　　要：为研究吗啡持续刺激对C57BL/6小鼠骨髓来源的树突状细胞成熟的影响。体外培养C57BL/6小鼠骨髓来源的树突状细胞,在培养过程中给予吗啡刺激,应用流式细胞术检测细胞表型CD11c、Ia、CD86的变化。吗啡干预各组树突状细胞中细胞表型CD11c、Ia、CD86表达较对照组显著升高。吗啡持续刺激能够促进小鼠树突状细胞的成熟。To investigate the effects of sustained morphine stimulation on the maturation of bone marrow derived dendritic cell（DC） and the expression of morphine receptor in mice.the primary C57BL/6 mouse bone marrow-derived dendritic cells of（4～6 weeks） were cultured in vitro,for 4 weeks and the cultivated cells were collected at 7 day after cultivation.The corresponding fluorescent antibody was then added.The changes of cell phenotypes of CD11c,Ia,CD86 molecules were detected by flow cytometry.The cells were collected at 7 th day and then the corresponding fluorescent antibody was added.The experimental result showed that the expressions of CD11c,Ia and CD86 of LPS＋morphine groups of experiment were significantly increased compared with those of blank control and LPS control group（P0.01）,but there were no significant differences between the LPS＋morphine groups（P0.05）.It suggests that sustained morphine stimulation can promote the maturation of mouse bone marrow-derived dendritic cells.

关 键 词：吗啡 树突状细胞 阿片受体 细胞表型 

分 类 号：R392.6[医药卫生—免疫学]