基于环氧合酶抑制作用的人工麝香质量评价方法研究  

Quality evaluation of artificial musk based on its inhibitory effect on cyclooxygenase-2

作　　者：罗云[1,2,3] 金城[1] 周健 温瑞卿[1,3] 李兴丰[4,5] 李瑞生[6] 杨明[2] 肖小河[1] 

机构地区：[1]解放军第三〇二医院解放军中药研究所,北京100039 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004 [3]成都中医药大学药学院,四川成都610075 [4]解放军第三二医院解放军中药研究所,北京100039 [5]湖南中医药大学药学院,湖南长沙410208 [6]解放军第三〇二医院实验动物中心,北京100039

出　　处：《药学学报》2011年第4期438-442,共5页Acta Pharmaceutica Sinica

基　　金：国家杰出青年基金资助项目(30625042);国家“重大新药创制”科技重大专项资助项目(2009ZXJ0904-057,2009ZX09502-022)

摘　　要：采用酶免疫(EIA)法,测定和计算人工麝香对环氧合酶Ⅱ(COX-2)的抑制率,构建人工麝香对COX-2抑制作用的量效关系,为建立人工麝香的体外抗炎效价测定方法提供研究基础。结果显示,人工麝香对COX-2具有明显抑制作用,半数抑制浓度(IC50)为2.26 mg·mL-1,在0.31～20.0 mg·mL-1内,具有明显的量效关系。结果表明,该方法快速、灵敏、准确、重现性好,可用于建立人工麝香抗炎生物效价检测方法,同时本文也为人工麝香的生物活性评价提供了新的研究思路和方法。The inhibitory ratio（I %） of artificial musk on cyclooxygenase-2（COX-2） was determined by enzyme immunoassay（EIA）.The dose-effect relationship between concentrations of artificial musk and I % was established.It was found that artificial musk had obvious inhibitory action on COX-2.The concentration for 50% of maximum inhibitory effect（IC50） was about 2.26 mg·mL-1.There was a good relationship between the logarithm concentrations of artificial musk and I % when the concentrations of artificial musk ranged from 0.31-20.0 mg·mL-1.The results indicated that this EIA method could be applied to evaluate the anti-inflammatory activity of artificial musk quickly,conveniently,sensitively and exactly.This paper provided a novel method and foundational research for the bioassay of artificial musk.

关 键 词：人工麝香 环氧合酶-2 酶免疫测定 抗炎 生物评价 

分 类 号：R917[医药卫生—药物分析学]