三维重建胫骨前肌在评估两种压力模式对大鼠深部组织损伤中的应用  

APPLICATION OF THREE-DIMENSIONAL RECONSTRUCTION OF TIBIALIS ANTERIOR MUSCLE IN EVALUATION OF RAT DEEP TISSUE INJURY UNDER TWO PRESSURE TYPES

作　　者：彭佳华[1] 王维平[1] 刘江辉[1] 黄勇[1] 郑光辉[1] 王淮玲[1] 王洁[1] 

机构地区：[1]中山大学附属第一医院急诊科,广州510080

出　　处：《中国修复重建外科杂志》2011年第4期436-441,共6页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的应用大鼠受压胫骨前肌连续切片获得的二维图像进行整体及损伤区三维重建,定量评估两种压力模式下的压力-损伤效应。方法取10～12周龄SD大鼠20只,雌雄不限,体重280～300g,随机分为实验组及对照组(n=10)。于一侧后肢取0.12cm2大小胫骨前肌部位,实验组给予间歇渐变性压力8.0～21.3kPa,对照组给予持续性压力13.3kPa。每施压2h间歇30min为1个周期,重复3个周期后终止施压,观察大鼠一般情况。于施压完成后24h大体观察受压部位皮肤及胫骨前肌,并取材连续4μm厚切片,HE染色后行二维图像采集,Image Pro Plus6.0图像采集分析系统进行全景图拼接及坏死、损伤区域轮廓定义划分,Photoshop8.0.1绘图软件图像配准,输入Mimics10.1软件进行三维重建,并获得胫骨前肌、损伤区三维图像及各自体积、表面积。结果两组动物均存活至实验完成,施压完成后24h时皮肤均无溃疡发生,受压皮肤和胫骨前肌均有不同程度水肿及压痕,其中对照组皮肤出现不褪色红斑。两组胫骨前肌标本共获994个切面,三维重建显示对照组损伤较明显,范围广且穿透于整个胫骨前肌,并沿胫骨骨面分布;实验组损伤区域较小,宽度与施压面积相似。两组胫骨前肌体积和表面积差异无统计学意义(P>0.05);而对照组损伤区体积和表面积均较实验组大,差异均有统计学意义(P<0.05)。结论三维重建可评估完整组织内病理变化,为力学性能在受压组织内的分布提供直观依据;与持续性压力比较,间歇渐变性压力可以减少深部组织损伤。Objective To quantitatively evaluate the effect of 2 types of pressures induced injury by using threedimensional（3D） reconstruction of rats loaded tibialis anterior muscle from two-dimensional（2D） image of serial histological sections.Methods Twenty female or male Sprague Dawley rats,aged 10-12 weeks and weighing 280-300 g,were randomly divided into experimental group（n=10） and control group（n=10）.The random side of tibialis anterior muscle was given intermittent gradient（8.0-21.3 kPa） and sustained（13.3 kPa） pressure in 0.12 cm2 area in experimental group and control group,respectively;the experiment was terminated and the general condition of rats was observed after 3 cycles,and a single cycle included 2 hours of compression and 30 minutes of release.The general observations of pressed skin and tibialis anterior muscle were done after 24 hours of pressure relief,and the tibialis anterior muscle was harvested integrally from the loaded side,then made into interval 4 μm serial sections.After HE staining,2D images were obtained.Necrosis and injury areas were distinguished by Image Pro Plus（IPP） 6.0 software and image registration was conducted by Photoshop 8.0.1 after 2D panorama images acquired by digital microscope（× 40） and IPP mosaic software.3D reconstruction was established via data processing using Mimics 10.1 software so as to get the volume,the surface area,and 3D images of the whole piece of tibialis anterior muscle and injury areas respectively.Results All rats of 2 groups survived till experiment terminated and no skin ulcers occurred after 24 hours.Edema and indentation were observed on press side skin and tibialis anterior muscles of 2 groups,fadeless maroon area was observed in control group.A total of 994 slices were obtained from 20 samples of tibialis anterior muscles.3D images suggested that injury of control group was severe,which penetrated the whole piece of tibialis anterior muscle and expanded along the tibia bony prominence.By contrast,injury of experiment

关 键 词：三维重建 胫骨前肌 深部组织损伤 压力性溃疡 间歇渐变性压力 持续性压力 大鼠 

分 类 号：R632.1[医药卫生—外科学]