水稻白条叶突变体(st10)的遗传分析与基因定位  

Genetic Analysis and Molecular Mapping of a White-stripe Leaf Mutation in Rice

作　　者：何颖红[1] 邹国兴[1,2] 饶玉春[1] 胡江[1] 刘坚[1] 高振宇[1] 郭龙彪[1] 朱丽[1] 钱前[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,杭州310006 [2]江西省农业科学院水稻研究所,南昌330200

出　　处：《分子植物育种》2011年第2期136-142,共7页Molecular Plant Breeding

基　　金：浙江省自然科学基金(Y3100357);国家转基因生物新品种培育重大专项研究计划(2008ZX08001-002);国家重点基础研究发展计划(2009CB118500);国家自然科学基金(30871513)共同资助

摘　　要：在水稻甲基磺酸乙酯(Ethylmethane Sulphonate,EMS)诱变突变体库中,发现了一个以粳稻品种日本晴(Nipponbare)为背景的白条叶突变体,该突变体叶片在2-3叶期出现纵向白条的突变性状,此后随着植株的生长逐渐弱化,至抽穗期叶片颜色基本恢复正常,只有叶脉仍呈白色。白条性状受温度影响,高温时性状明显,所有突变个体出现白穗现象。低温条件下,白条较窄,颜色不明显,穗的颜色正常。以培矮64s为母本,突变体st10为父本构建分离群体,遗传分析结果表明该突变性状由一对单隐性核基因控制。利用F2分离群体中突变型植株将目的基因定位在第3染色体短臂上STR19和STR24两个分子标记之间150kb的范围内。本实验测定了24℃、28℃和32℃三个温度条件下的叶绿素含量,发现突变体st10在32℃条件下叶绿素a和叶绿素b的含量减少,28℃、24℃条件下变化不明显。叶绿素荧光数据分析结果显示白条叶突变体的最大量子产额低于野生型。We found a temperature-sensitive white-stripe leaf mutant st10 at a background of japonica variety Nipponbare which is induced by ethyl methane sulphonate （Ethylmethane Sulphonate, EMS）. It appears white-stripe at 2-leaf or 3-leaf stage. The white-stripe becomes narrow and veins show white at early panicle formation and heading stage.The leaf colour is affected by temperature. When grown at higher temperature, it displayed white-stripe obviously, even white with grain. However, the white-stripe is narrow and the grain is normal at lower temperature. St10 is shorter than Nipponbare, but the tillering is not significant different with it. The result of genetic analysis revealed that white-stripe phenotype was controlled by a single recessive nucleic gene. The F2 population is generated from a across between peiai64s （a male sterility line） and st10. St10 gene was mapped in 150 kb region between marker STR19 and marker STR24 on chromosome 3. We tested the chlorophyll contents of three temperatures, 24℃, 28℃ and 32℃. Chlorophyll b and chlorophyll a are significantly reduced at 32℃, however, they are did not change significantly at 24℃ and 28℃. Chlorophyll Fluorescence data show that photosynthetic capacity of the mutant is lower than wild type. The decrease is probably due to a decrease of chlorophyll contents, which is an action of the temperature regulating gene expression.

关 键 词：水稻突变体 白条叶 基因定位 

分 类 号：S511[农业科学—作物学]