高直链淀粉玉米amylose-extender基因功能标记的开发及应用  

Development and Utilization of amylose-extender Functional Marker in High-amylose Maize

作　　者：宁丽华[1] 陈亭亭 刘怀华[1] 刘旭[1] 马侠[1] 崔德周[1] 姜川[1] 张华[1] 王莉雯[1] 李德涛 陈化榜[1] 

机构地区：[1]作物生物学国家重点实验室,山东省作物生物学重点实验室,山东农业大学农学院,泰安271018

出　　处：《分子植物育种》2011年第2期185-189,共5页Molecular Plant Breeding

基　　金：国家自然基金项目(31071424);山东省良种工程重大课题鲁农良种字(2008)共同资助

摘　　要：直链淀粉的含量是玉米淀粉品质的重要指标,受隐性核基因amylose-extender(ae)控制。生产中直链淀粉含量的测定费时、费力,因此高直链淀粉玉米的改良需要寻找快速、简便、准确的基因检测方法。本实验对高直链淀粉玉米材料和普通玉米材料ae基因的cDNA进行了测序,结果显示高直链淀粉材料cDNA序列第9外显子(84bp)全部缺失。利用该缺失设计的玉米ae基因的功能标记(ae-InDel),能够检测出纯合基因型个体(AeAe和aeae)和杂合基因型个体(Aeae),该共显性标记能有效用于高直链淀粉玉米的分子标记辅助育种。Amylose in maize, a recessive trait conferred by nuclear gene （ae）, is one of the most important qualities of maize starch. Maize breeders have an interest in gaining access to a quick, precise and simple method for disti- nguishing between high amylose miaze and normal ones. We sequenced cDNAs from high-amylose and normal corn inbred lines and designed a co-dominant functional marker ae-InDel. Our result showed that the ae-InDel could distinguish aeae homozygous genotype from homozygous genotype AeAe and heterozygous genotype Aeae. The result suggested that the ae-InDel could be effectively applied in marker assisted selection for increasing amylose content in maize.

关 键 词：玉米 高直链淀粉 功能标记 

分 类 号：S513[农业科学—作物学]