LPS介导滋养层细胞TLR4信号传导通路对HBD-2表达的影响  

Effects of LPS-TLR4 signaling pathways on expression of HBD-2 in trophoblastic cells

作　　者：颜露春[1] 黄星[1] 游琼[2] 徐群香[1] 

机构地区：[1]广西医科大学第一附属医院妇产科,南宁530021 [2]广东医学院附属医院心血管内科,湛江524000

出　　处：《免疫学杂志》2011年第3期243-245,249,共4页Immunological Journal

基　　金：广西研究生教育创新项目(2009105981002M230);广西科技厅自然科学基金项目(桂科自0832115)

摘　　要：目的研究LPS体外刺激滋养层细胞是否诱导表达HBD-2,并进一步探讨该表达与TLR4信号传导通路的关系。方法建立不同孕周的滋养层细胞原代培养体系,应用TLR4阻断剂预处理滋养层细胞30 min前后,给予不同质量浓度的LPS(25、50、100、200、400 ng/ml)作用72 h,采用SYBR GreenⅠ实时荧光定量RT-PCR技术检测滋养层细胞HBD-2 mRNA的表达。结果 1)LPS能诱导滋养层细胞HBD-2 mRNA表达,且这种表达与其具有浓度和时间依赖性;当质量浓度为200 ng/ml,刺激24 h时,HBD-2 mRNA的相对表达量最高;2)TLR4阻断剂能抑制LPS对滋养层细胞表达HBD-2 mRNA的诱导作用,与未阻断之前相比差异具有统计学意义(P<0.01)。结论 LPS可能通过TLR4信号传导通路诱导滋养层细胞表达HBD-2 mRNA,将为宫内感染防治提供新靶点。We aimed to identify whether Lipopolysaccharide（LPS） could upregulate the expression of human beta-defensin-2（HBD-2） in trophoblastic cells,and further discuss the role of LPS-TLR4 signaling pathways in this procedure.We firstly establish a stable trophoblastic primary culture cell system.The trophoblastic cells were pretreated with TLR4 blocker or not,then stimulated with LPS at concentrations of 25,50,100,200,400 ng/ml respectively.At last,we detected the expression of HBD-2mRNA by SYBR using Green fluorescent quantitativeⅠ real-time PCR in trophoblastic cells.We found LPS promoted expression of HBD-2 in trophoblastic cells in a dose-and time-dependent manner;the optimal concentration is 200 ng/ml,and the maximal expression of HBD-2 mRNA appeared at 24 hours after the LPS stimulation.Otherwise,TLR4 blockers could inhibit the LPS-induced HBD-2 expression in trophoblastic cells.We concluded that LPS can enhance the activation of HBD-2 mRNA by TLR4 signaling pathways in trophoblastic cells.This founding will benefit the therapy for intrauterine infection.

关 键 词：人Β-防御素-2 Toll样受体4 宫内感染 脂多糖 SYBR GreenⅠ实时荧光定量RT-PCR 

分 类 号：R392.33[医药卫生—免疫学]