雌激素受体α启动子区甲基化与冠状动脉粥样硬化的关系  

Association of methylation of the estrogen receptor-α gene with coronary heart disease

作　　者：靳松[1] 沈干[1] 胡世莲[1] 马礼坤[1] 吴蕾[1] 陈尹[1] 徐婷娟[1] 

机构地区：[1]安徽医科大学附属省立医院老年医学科,安徽合肥230001

出　　处：《中国老年学杂志》2011年第7期1102-1104,共3页Chinese Journal of Gerontology

基　　金：2008年度安徽省科技攻关项目(No.08010302180)

摘　　要：目的探讨雌激素受体(ER)-α基因甲基化状态在冠状动脉粥样硬化发生中的作用。方法采用甲基化特异性PCR(MSP)技术检测40例冠心病患者外周血ER-α基因启动子区甲基化状态,以20例健康成人为对照组。采用Real-time RT-PCR检测ER-α基因的mRNA表达水平。结果实验组ER-α基因启动子区甲基化阳性率显著高于对照组分别为77.5%和55%;对照组人群ER-α基因的mRNA表达水平是实验组的2.57倍。结论在冠状动脉粥样硬化患者外周血中存在ER-α基因的高甲基化和低表达状态,可能参与其发生发展过程。Objective To discuss the relationship between methylation of ER-α gene CpG island and coronary atherosclerosis.Methods 60 cases including 40 coronary atherosclerosis patients and 20 healthy control subjects.Methylation-specific polymerase chain reaction was used to detect promoter methylation status of the ER-α and the mRNA expression were investigated by real time RT-PCR.Results The methylation of ER-α gene was increased in coronary atherosclerotic patients compared to healthy people,while mRNA expression of ER-α in controlled group was 2.57 times as much as that of coronary atherosclerotic patients.Conclusions The aberrant methylation and low expression of ER-α may contribute to the development of coronary atherosclerosis.

关 键 词：雌激素受体 冠心病 甲基化 

分 类 号：R541[医药卫生—心血管疾病]