兔骨关节炎模型血清炎症指标的动态观察  

Dynamic observation of inflammation index in osteoarthritis rabbit model

作　　者：庄超[1] 刘瑞平[1] 徐南伟[1] 王黎明[2] 徐燕[2] 

机构地区：[1]南京医科大学附属常州第二人民医院骨科,江苏常州213003 [2]南京医科大学附属南京第一医院骨科,江苏南京210006

出　　处：《南京医科大学学报（自然科学版）》2011年第3期369-373,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金(06NMUZ045)

摘　　要：目的:通过观察兔骨性关节炎(osteoarthritis,OA)模型血清中相关炎症指标的连续动态改变,探讨其在反映OA病程进展中的价值。方法:70只雄性新西兰大白兔随机分成OA组和对照组,每组35只。OA组实验兔左膝关节腔注射2%浓度的木瓜蛋白酶0.5 ml,建立OA模型;对照组左膝关节腔注射等量无菌生理盐水。在建模后第1、2、4、6、8、10、12周分别取实验兔动脉血检测血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平,取兔膝关节标本行病理观察并进行Mankin’s评分。结果:OA组出现关节软骨变薄、基质分解破坏、滑膜增生等炎症及退行性改变,并随观察时间的延长而进一步发展;血清IL-1β、TNF-α和IL-6水平明显升高,与对照组相比有显著性差异,结果同Mankin’s评分一致。结论:血清IL-1β、TNF-α和IL-6水平与OA病变程度平行,可用来提示OA临床病情发展。Objective：To evaluate the function of inflammation index in osteoarthritis by continuous dynamic observation.Methods：Seventy New Zealand white rabbits were randomly and equally divided into an osteoarthritis group and a normal control group.Osteoarthritis was induced by injecting 0.5 ml of 2% papain into left knee joints of rabbit,and the same volume of sterile saline solution was injected into left knee joints control group.The serum IL-1β,TNF-α and IL-6 levels were detected on the 1,2,4,6,8,10 and 12 week after injection.Then the rabbits were sacrificed and the knee joints were harvested and studied in pathohistological as well as Mankin scores.Results：Compared with the control group,degenerated changes were observed in OA group,such as thinner articular cartilage,fibrillation and destroyed cartilage matrix,as well as inflammation,proliferation and degeneration of the synovial tissue.All these symptoms got worse with prolonged observation time.The serum IL-1β,TNF-α and IL-6 levels increased in OA group,which were coincided with Mankin scores.Conclusion：Serum IL-1β,TNF-α and IL-6 levels increased with OA process and indicated the progression of disease.

关 键 词：骨性关节炎 兔模型 炎症指标 动态观察 

分 类 号：R684.3[医药卫生—骨科学]