2型糖尿病患者皮下、大网膜脂肪组织脂联素mRNA表达变化及意义  

Expression and significance of adiponectin mRNA in subcutaneous and omental adipose tissue of type 2 diabetic patients

作　　者：李倩倩[1] 蒋秀琴[1] 李慧敏[1] 缪珩[1] 

机构地区：[1]南京医科大学第二附属医院内分泌科,江苏南京210011

出　　处：《南京医科大学学报（自然科学版）》2011年第3期404-409,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学重点课题基金资助(07NMUZ014)

摘　　要：目的:观察2型糖尿病患者皮下及大网膜脂肪组织脂联素(adiponectin)表达水平的变化,及其与血清脂联素、体质指数(BMI)、腰围(WC),腰/臀比值(WHR)、收缩压(SBP)、舒张压(DBP)、空腹血糖(FPG)、糖化血红蛋白(HbA1c)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、尿酸(UA)等相关指标的关系,探讨脂联素在2型糖尿病患者发生胰岛素抵抗中的作用。方法:用半定量RT-PCR方法检测23例2型糖尿病合并肥胖患者(DM+FAT)、23例2型糖尿病患者(DM)、33例非糖尿病肥胖患者(control+FAT)、23例非糖尿病患者(control)腹部皮下及大网膜脂肪组织脂联素mRNA的表达水平,并测量身高、体重、腰围、臀围、血压、FPG、HbA1C、血脂、尿酸、血清脂联素,并计算BMI、WHR。结果:①糖尿病肥胖组SBP、DBP、FPG、HbA1c、TG、LDL-C、尿酸均高于对照肥胖组,HDL-C、皮下及大网膜脂肪组织脂联素mRNA表达量、血清脂联素均低于对照肥胖组(P<0.01)。两组的大网膜脂联素表达量低于其皮下表达量(P<0.01);②糖尿病非肥胖组的FPG、HbA1c、TG、尿酸均高于对照非肥胖组,HDL-C、皮下及大网膜脂肪组织脂联素mRNA表达量、血清脂联素均低于对照非肥胖组(P<0.01)。两组的大网膜脂联素表达量低于其皮下表达量(P<0.01);③糖尿病肥胖组的BMI、WHR、SBP、DBP、FPG、HbA1c、TG、尿酸均高于糖尿病非肥胖组,大网膜脂肪组织脂联素mRNA表达量、血清脂联素均低于糖尿病非肥胖组(P<0.01);④直线相关分析显示,糖尿病肥胖组的网膜脂联素mRNA的表达水平与BMI、FPG(P<0.01)、WHR、WC(P<0.05)呈负相关,与血清脂联素水平呈正相关(P<0.01)。结论:2型糖尿病合并肥胖患者大网膜脂肪组织脂联素mRNA表达显著降低,血清脂联素水平显著降低;BMI、WC、WHR、FPG等临床指标可间接反映胰岛素抵抗程度。Objective：To observe the adiponectin mRNA expression in subcutaneous and omental adipose tissue of type 2 diabetic patients and their relation with serum adiponectin,body mass index（BMI）,waist circumference（WC）,waist hip ratio（WHR）,blood pressure,fasting plasma glucose（FPG）,HbA1c,blood lipids and uric acid（UA）.Methods：Twenty-three T2DM patients with obesity,23 T2DM patients,33 obesity patients and 23 controls were selected.The adiponectin mRNA expression level in adipose tissue was assayed by RT-PCR.The level of serum adiponectin was measured with ELISA kit.FBG,triglyceride（TG）,total cholesterol（TC）,high-density lipoprotein cholesterol（HDL-C）,low-density lipoprotein cholesterol（LDL-C）,HbA1c,UA of all subjects were determined and their blood pressure,height,weight,waist circumference,hip circumference were measured to calculate BMI,WHR.Results：①The SBP,DBP,FPG,HbA1c,TG,LDL-C,UA were significantly higher,and serum adiponectin,HDL-C and adiponectin mRNA expression level in both subcutaneous and omental adipose tissue were significantly lower in diabetic obesity group than in obesity group（P 〈 0.05）,the adiponectin mRNA expression level in omental were significantly lower than in subcutaneous adipose tissue in both groups（P 〈 0.01）.②The FPG,HbA1c,TG,UA were higher,serum adiponectin,HDL-C and adiponectin mRNA levels expressed in both subcutaneous and omental adipose tissue were lower in diabetic group without obesity than in control group without obesity（P 〈 0.05）,the adiponectin mRNA expression level in omental was lower than in subcutaneous adipose tissue in both groups（P 〈 0.01）.③The BMI,WHR,SBP,DBP,FPG,HbA1C,TG,UA were higher,serum adiponectin and adiponectin mRNA level expressed in both subcutaneous and omental adipose tissue were lower in diabetic obese group than in diabetic group without obesity（P 〈 0.05）.④The adiponectin mRNA expression level in omental adipose tissue of diabetic obesity group was negatively correlated to BMI,WHR,

关 键 词：脂联素 2型糖尿病 体质指数 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]