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作 者:张瑞玖[1,2] 陈有君[3] 蒙美莲[1] 郦海龙[1] 周长艳[1] 冯琰[1,2]
机构地区:[1]内蒙古农业大学农学院,呼和浩特010019 [2]河北省张家口市农业科学院,张家口075000 [3]内蒙古农业大学生命科学院,呼和浩特010018
出 处:《Agricultural Science & Technology》2010年第11期65-67,共3页农业科学与技术(英文版)
基 金:Supported by National Key Technology Research and Development Program(2007BAD49B03-1)~~
摘 要:The effects of nitrogen on physiological and biochemical characteristics of potato cultivar Zihuabai under NaCl stress were studied in culture condition.The results show that,under NaCl stress,the contents of proline,chlorophyll and protein and root system vitality first increase and then decrease with the increase of nitrogen level,and reach the top under 4.17 mmol/L NH4NO3 level.Wherein,the contents of chlorophyll,protein and root system vitality are respectively 69.88%,13.07% and 59.29% higher than that of the control under 4.17 mmol/L NH4NO3 level;the activities of superoxide dismutase(SOD)and peroxidase(POD)increase generally under NaCl stress with the increase of nitrogen level,and reach the peaks [111.83 U/g and 25.467 U/(g·min)],which are 37.73% and 35.46% higher than that of control,at 6.25 mmol/L NH4NO3 level.以马铃薯紫花白品种为试验材料,采用组织培养方法,在离体条件下研究了NaCl胁迫下氮素对马铃薯生理生化特性的影响。主要结果如下:NaCl胁迫下马铃薯叶片脯氨酸含量、叶绿素含量、可溶性蛋白含量及根系活力随氮水平的提高表现为先增加后降低的趋势,在(N2)4.17mmol/LNH4NO3水平下达到最大值,分别为521.21μg/g、1.96mg/g、11.08mg/g、5619.44μg/(g.h),其中叶绿素含量、可溶性蛋白含量及根系活力在N2水平下显著的高于对照,分别较对照高69.88%、13.07%和59.29%;NaCl胁迫下马铃薯叶片SOD和POD的活性随氮水平的增加逐渐上升,且在(N3)6.25mmol/LNH4NO3水平下达到最大值,其值分别为111.83U/g和25.467U/(g.min),较对照增加了37.73%和35.46%。
关 键 词:POTATO NaCl stress Nitrogen level Physiological and biochemical characteristics
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