Sterile Germination of Dendrobium chrysotoxum Seeds and Rapid Propagation of Its Plantlets  

鼓槌石斛种子萌发培养与小苗组培快繁技术研究(英文)

作　　者：蓝玉甜 刘世勇 罗玉婷 黄岚 李柱林 韦小路 

机构地区：[1]广西雅长兰科植物国家级自然保护区管理局,广西乐业533209

出　　处：《Agricultural Science & Technology》2010年第11期89-91,共3页农业科学与技术（英文版）

摘　　要：[Objective] This study was to produce plant seeds on a large scale via sterile germination of the capsules of Dendrobium chrysotoxum and rapid propagation technique.[Method] A large amount of protocorm-like bodies produced from the sterile germination of D.chrysotoxum capsules,were transferred to four different kinds of bud induction media to obtain the optimal media suitable for plantlet differentiation and growth;and then with N6 as basic medium,1.0,1.5 and 2.0 mg/L of NAA and IAA were tested to obtain the optimal media suitable for rooting.[Results] On the medium of MS appended with 1 mg/L 6-BA ＋10% banana puree ＋ 20 g/L sucrose ＋6 g/L agar＋1 g/L AC,seed germination rate was up to 90%.The optimal medium for differentiation of D.chrysotoxum protocorm-like bodies was N6＋ 2 mg/L NAA ＋ 0.5 mg/L 6-BA ＋10% banana puree ＋ 20 g/L sucrose ＋ 0.5 g/L peptone ＋ 5.8 g/L agar ＋0.5 g/L AC,grown from which the plantlets were even and orderly in height;and the optimal medium for rooting was N6＋1.5 mg/L NAA ＋10% banana puree ＋ 20 g/L sucrose ＋ 5.8 g/L agar ＋1 g/L AC,grown from which the plantlets developed more,robust and orderly roots,and their leaves were in dark green color.[Conclusion] Our results provided reference for the rapid propagation of D.chrysotoxum.[目的]利用鼓槌石斛蒴果,通过无菌播种萌发发育成苗及小苗快繁技术试验研究,从而获得大量种苗。[方法]用鼓槌石斛种子通过无菌播种获得大量原球茎,分别转接到4组不同的诱导芽培养基上,筛选出鼓槌石斛最适合小苗分化和成长的培养基,以其设置以N6为基本培养基,添加浓度均为1.0、1.5、2.0mg/L的NAA和IAA,筛选最适合鼓槌石斛小苗生根壮苗培养基。[结果]将鼓槌石斛种子在MS+6-BA1mg/L+10%香蕉汁+20g/L蔗糖+6g/L琼脂+1g/LAC培养基上进行培养种子,种子萌发率达90%以上;最利于原球茎分化的培养基为N6+NAA2mg/L+BA0.5mg/L+10%香蕉汁+20g/L蔗糖+0.5g/L牛肉蛋白胨+5.8g/L琼脂+0.5g/LAC,培养出的苗整齐度高,均匀;最佳生根壮苗培养基为N6+NAA1.5mg/L+10%香蕉汁+20g/L蔗糖+5.8g/L琼脂+1g/LAC,苗生根数多、粗壮、整齐,叶浓绿。[结论]该研究结果为鼓槌石斛快速繁育技术提供了参考。

关 键 词：Dendrobium chrysotoxum Sterile germination Plantlet culture Rapid propagation 

分 类 号：S682.31[农业科学—观赏园艺]