依达拉奉对大鼠急性脊髓损伤后神经细胞凋亡的影响  

Edaravone Reduces Cell Apoptosis after Acute Spinal Cord Injury in Rats

作　　者：武志坚[1] 岳志健[1] 张煜辉[1] 

机构地区：[1]第二军医大学长海医院神经外科,200433

出　　处：《中国临床神经科学》2011年第2期183-188,共6页Chinese Journal of Clinical Neurosciences

摘　　要：目的:观察依达拉奉对大鼠急性脊髓损伤后细胞凋亡的影响,探讨脊髓保护的作用机制。方法:120只SD雄性大鼠,Allen法建立脊髓损伤模型,随机分为依达拉奉组、假手术组和对照组(均n=40)。依达拉奉组给予依达拉奉10mg·kg^(-1),每日2次腹腔注射,连续6d;对照组给予等量同次数的生理盐水腹腔灌注;假手术组仅行椎板切除,不损伤脊髓,不给药。在伤后第1、7、14、28天观察各组大鼠活动情况行BBB评分,取受伤脊髓节段检测抑制羟自由基能力、caspase-3蛋白表达、原位脱氧糖核苷酸末端转移酶介导的原位末端标记法(TUNEL法)标记凋亡细胞。结果:依达拉奉组抑制羟自由基能力明显高于假手术组和对照组(P<0.05);依达拉奉组caspase-3与对照组比各时间点的表达明显降低(P<0.05);依达拉奉组与对照组比各时间点凋亡细胞显著减少(P<0.05)。结论:依达拉奉能减少急性脊髓损伤部位的羟自由基,下调caspase-3表达,抑制脊髓神经细胞凋亡,对继发性脊髓损伤有保护作用。Aim：To investigate the effect of edaravone on cell apoptosis after spinal cord injury and explore its protective effect and action mechanism.Methods：120 SD rats were randomly divided into three groups： sham,control and edaravone-treating groups.There were 40 rats in each group.SD rat model of acute spinal cord injury were established by Allen＇s weight dropping.The change of nerve functions and hydroxy radical repressive activity were assessed.The expression of caspase-3 was tested with immunohistochemistry and the apoptosis was labeled by TUNEL method.The differences and effects of each group were compared. Results：The hydroxy radical repressive activity in the spinal cord tissue at day 1,day 7,and day 14 after acute spinal cord injury were significantly different between the sham-operated group and the control group （P0.05）.The caspase-3 protein level was lower in the edaravone group than that in the control group. Tunel-positive cells were significantly decreased at day 7（P0.05）.Conclusion：Edaravone can decrease hydroxy radical repressive activity and the expression of caspase-3,and inhibit neuron and glial cell apoptosis in the rats after spinal cord injury,which may play a significant neuroprotective role in the secondary spinal cord injury.

关 键 词：依达拉奉 脊髓损伤 CASPASE-3 原位末端标记法 凋亡 

分 类 号：R651.2[医药卫生—外科学] Q189[医药卫生—临床医学]