桃红四物汤对寒凝血瘀模型大鼠卵巢血管舒-缩因子的影响  

Effect of Taohongsiwu Decoction on model rat of blood stasis due to accumulation of cold in vasorelaxation-vasoconstriction factors

作　　者：成秀梅[1] 杜惠兰[1] 李丹[1] 路帅[1] 

机构地区：[1]河北医科大学中西医结合学院,石家庄050091

出　　处：《中药药理与临床》2011年第1期5-7,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(30973766)

摘　　要：目的：观察桃红四物汤对寒凝血瘀模型大鼠卵巢血管舒-缩因子及卵巢功能的影响。方法：将大鼠置于0℃1℃冰水中造成寒凝血瘀大鼠模型,采用阴道涂片检测大鼠动情周期;采用放射免疫法测定血清E2、P、T水平;剖取卵巢,制成10%的匀浆,测定ET-1、NO、Ang-2、CGRP活性。结果：模型组大鼠动情周期延长,血清E2、P、T明显下降,卵巢组织匀浆ET-1、Ang-2活性升高,NO、CGRP活性降低,与正常组比较有统计学意义;桃红四物汤组动情周期缩短,血清E2、P、T明显升高,卵巢组织匀浆ET-1、Ang-2活性降低,NO、CGRP活性升高,与模型组比较有统计学意义。结论：桃红四物汤通过调节血管舒缩功能,改善生殖激素水平治疗寒凝血瘀型妇科疾病。Objective： To observe the effect of Taohongsiwu Decoction on model rat of blood stasis due to accumulation of cold in vasorelaxation-vasoconstriction factors and in ovarian function.Methods： The rats model of blood stasis due to accumulation of cold were induced by keeping in water at 01℃.The estrous cycle was detected by vaginal smear.The level of serum E 2,P,T were measured by radioimmunoassay.Rats’ ovaries were prepared for the solution of 10% homogenate to measure ET-1、NO、Ang-2、CGRP.Results： Compared with the normal group,The estrous cycle in model group was significantly prolonged and the level of serum E 2、P、T and the activity of NO、CGRP in ovary homogenate were significantly decreased.The concentrations of ET-1,Ang-2 in ovary homogenate were increased.After administration with Taohongsiwu Decoction,The estrous cycle was significantly decurtated and the level of serum E 2、P were increased.The activity of ET-1,Ang-2 in ovary homogenate were decreased.Meanwhile the activity of NO、CGRP in ovary homogenate were increased.There was positive correlation between P and NO,CGRP in ovary homogenate,a negative correlation between P and ET-1、Ang-2 in ovary homogenate.There was negative correlation between E 2 and ET-1 in ovary homogenate.Conclusion： The therapeutical effect of Taohongsiwu Decoction on gynecological desease may act through regulating vasomotor function and improving the level of reproductive hormone.

关 键 词：桃红四物汤 寒凝血瘀 生殖激素 血管舒缩因子 

分 类 号：R285.5[医药卫生—中药学]