柯里拉京对叔丁基过氧化氢损伤N9细胞的保护作用  

Protective effects of corilagin against tert-butyl hydroperoxide-induced cytotoxicity in N9 cells

作　　者：陈一燕[1] 陈崇宏[2] 

机构地区：[1]厦门大学附属第一医院药学部,厦门361003 [2]福建医科大学药学院药理学系,福州350004

出　　处：《中药药理与临床》2011年第1期16-18,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的：研究柯里拉京对活性氧诱导小鼠小胶质细胞N9氧化损伤的保护作用。方法：以叔丁基过氧化氢（tert-butyl hydroperoxide,t-BHP,0.6 mM）孵育24 h损伤N9细胞建立活性氧氧化应激模型;以柯里拉京（0.54μM）预孵育24 h进行干预,通过光镜观察细胞形态差异,MTT法检测细胞活力,罗丹明123染色法检测细胞线粒体膜电位（mitochondrial membrane potentials,MMP）,硫代巴比妥酸法测定丙二醛（malondialdehyde,MDA）含量等观察柯里拉京预处理的保护作用。结果：与氧化损伤模型组比较,柯里拉京预处理能明显提高t-BHP诱导损伤的N9细胞活力,改善损伤的N9细胞形态,显著抑制t-BHP引起的MMP降低和MDA升高。结论：柯里拉京能够抑制活性氧对N9细胞的氧化损伤,其保护作用可能与其抑制氧化应激引起线粒体功能损伤有关.Objective： To observe the protective effects of corilagin on tert-butyl hydroperoxide-induced toxicity murine microglia N9 cells.Methods： N9 murine microglia cells exposed to tert-butyl hydroperoxide（0.6mM） for 24 h were used as an in vitro model of oxidative stress.The antagonist effects of corilagin were observed by pretreatment with corilagin（0.54 μM） for 24 h prior to t-BHP challenge.Cell morphology was observed by phase contrast microscope,cell viability was determined by MTT method,the malondialdehyde（MDA） level was determined by thiobarbituric acid analysis and mitochondrial membrane potential（MMP） was measured by Rh123 fluorospectrophotometry.Results： Compared with the oxidative stress group,the cell viabilities of N9 cells pretreated with corilagin were improved,MDA lowered in the supernatants lowered and the MMP up-regulated significantly.Conclusion： Corilagin has protective action against tert-butyl hydroperoxide-induced toxicity in N9 cells,which might be mediated through the mitochondrial protective effect owing to its antioxidant property.

关 键 词：柯里拉京 叔丁基过氧化氢 N9细胞 氧化应激 

分 类 号：R285[医药卫生—中药学]