薯蓣皂苷对大鼠心室肌细胞钙离子通道的影响  

Effects of dioscin on calcium channels in rat ventricular cardiomyocytes

作　　者：张铭慧[1] 尹永强[1] 何海燕[1] 康毅[2] 娄建石[1] 

机构地区：[1]天津医科大学药理学教研室,天津300070 [2]天津医科大学中药药理实验室,天津300070

出　　处：《中药药理与临床》2011年第1期23-26,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家天然药物工程技术中心资助(200703)

摘　　要：目的:研究薯蓣皂苷对正常大鼠心室肌细胞L-钙电流(ICa,L)的影响,旨在探讨薯蓣皂苷对心肌缺血再灌注(I/R)损伤保护作用的可能机制。方法:酶解法分离大鼠单个心室肌细胞,应用全细胞膜片钳技术记录薯蓣皂苷低(10μg/ml)、中(20μg/ml)、高(30μg/ml)三个剂量对大鼠单个心室肌细胞ICa,L的影响。结果:薯蓣皂苷10μg/ml不影响ICa,L峰值,而20μg/ml和30μg/ml能使ICa,L峰值降低,抑制呈剂量依赖性;20μg/ml和30μg/ml薯蓣皂苷使I-V曲线上移,内向电流减小,但基本不改变曲线的形状。30μg/ml薯蓣皂苷使失活曲线向负电位方向变化,曲线左移,各剂量均对激活曲线无影响。结论:薯蓣皂苷通过促进ICa,L失活的过程抑制正常大鼠心室肌细胞I,减小I峰值,这可能是其抗缺血再灌所致心律失常的作用机制之一。Aim： To investigate the effect of dioscin on L-type calcium current（I Ca,L） of ventricular cardiomyocytes and explore the possible protective mechanism of dioscin against the ischemia/reperfusion（I/R） injury.Methods： Whole-cell patch clamp was used to record I Ca,L in single ventricular cardiomyocytes of rats.Results： In ventricular cardiomyocytes of rats,calcium peak current was decreased by 20～30μg/mldioscin in a concentration-dependent manner,while 10μg/ml had no effect on it.The current-voltage curve was shifted upwards by 20～30μg/mldioscin,which curve shape had no significant changes;The inactivation curve was shifted to more negative potentia1 by 30μg/ml dioscin.but the activation curve was not altered.Conclusion： Dioscin inhibits I Ca,L of ventricular cardiomyocytes by facilitating the inactivation of I Ca,L,which may partly attribute to the antiarrhythmic action in ischemia/reperfusion（I/R） injury.

关 键 词：薯蓣皂苷 心肌细胞 心肌缺血 钙通道 全细胞膜片钳技术 

分 类 号：R285.5[医药卫生—中药学]