秃疮花提取物对CCl4诱导的小鼠急性肝细胞微粒体损伤的影响  

Protection of Picranostigma leptopodum extraction against acute hepatic microsome injuries induced by carbon tetrachloride

作　　者：毛爱红[1] 王勤[2] 王廷璞[2] 夏晓慧[2] 魏娟[2] 王明辉[2] 

机构地区：[1]甘肃省医学科学研究院,兰州730050 [2]兰州大学生命科学学院,兰州730000

出　　处：《中药药理与临床》2011年第1期73-75,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：甘肃省兰州科技局科研基金资助课题

摘　　要：目的:探讨研究秃疮花提取物(DLE)对CCl4诱导的小鼠急性损伤肝细胞微粒体的影响。方法:小鼠灌胃给予不同剂量的DLE,连续2周。最后一次给药24h后CCl4造成急性肝损伤。制备肝细胞微粒体悬液,检测抗氧化酶SOD、CAT、GSH-Px的活性;测量肝细胞微粒体悬液中的MDA水平;观察NADPH-细胞色素C(P450)还原酶活性及ATPase活性的变化。结果:与模型组相比,不同剂量的DLE给药组肝细胞微粒体悬液中MDA含量显著减少;抗氧化酶SOD、GSH-Px活性显著增加;肝细胞微粒体NAD-PH-细胞色素C(P450)还原酶活性降低,Na+K+-ATPase和Ca2+-ATPase活性显著上升。结论:DLE对CCl4诱导的小鼠肝微粒体损伤具有很好的保护作用。其作用机理与DLE具抗氧化和自由基活性清除有关。Objective： This study examined the effect of DLE on hepatic microsome injury,from the aspect of lipid peroxidation after CCl 4 administration in mice.Methods： The activities of antioxidant enzymes of superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-Px） in liver plasma and Na ＋,K ＋-ATPase,Ca 2＋-ATPase in hepatic microsome suspension were detected respectively by its kits.Spectrophotography measured the activities of NADPH-cytochrome C（P450） reductase in microsome.The levels of malondialdehyde（MDA） in hepatic microsome suspension were measured by TBA method.Results： The activities of SOD and GSH-Px were significantly increased by DLE pretreatment.The pretreatment of DLE not only markedly increased the activities of Na ＋,K ＋-ATPase and Ca 2＋-ATPase,it also partially prevented the activities of NADPH-cytochrome C（P450） reductase and the levels of MDA from increasing.These results indicated that DLE has the protective effect against the experimental liver injury induced by carbon tetrachloride in mice.Conclusion： The DLE could stimulate hepatic antioxidant enzymes activities and remove free radicals generated by CCl 4 metabolized.

关 键 词：秃疮花 急性肝损伤 肝细胞微粒体 

分 类 号：R285.5[医药卫生—中药学]