溃结灵对溃疡性结肠炎大鼠结肠粘膜pMEK1/2、pERK1/2蛋白水平动态变化的影响  

The effect of Kuijieling on dynamic changes of pMEK1/2 and pERK1/2 protein expression in ulcerative colitis rats

作　　者：黄秋凌[1] 李燕舞[1] 王汝俊[1] 

机构地区：[1]广州中医药大学脾胃研究所,广州510405

出　　处：《中药药理与临床》2011年第1期81-83,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(No:30772754);广东省中医药局课题(No:2007047)

摘　　要：目的:观察溃结灵对TNBS法UC大鼠模型结肠粘膜pMEK1/2、pERK1/2蛋白水平的影响。方法:UC大鼠模型采用TNBS法,提取结肠粘膜全细胞蛋白,运用蛋白免疫印迹(Western blot)方法对pMEK1/2和pERK1/2的蛋白表达水平进行检测。结果:与正常组比较,3d时模型组pMEK1/2、pERK1/2蛋白相对含量略有升高,7d时pMEK1/2、pERK1/2均持续增高,14d时pMEK1/2、pERK1/2均明显增高。与模型组比较,3d时溃结灵组和SASP组pMEK1/2,pERK1/2蛋白相对含量均略有增高,7d时溃结灵组及SASP组pMEK1/2、pERK1/2均持续增高(P<0.05,P<0.01,P<0.05),14d时溃结灵组及SASP组均明显高于模型组(P<0.05,P<0.01)。结论:溃结灵对TNBS法UC大鼠模型结肠粘膜pMEK1/2、pERK1/2蛋白表达有上调作用,提示MEK/ERK信号通路可能是溃结灵修复UC大鼠模型肠道损伤粘膜的途径之一。Objects： To observe the effect of kuijieling on dynamic changes of pMEK1/2 and pERK1/2 protein expression in ulcerative colitis rats.Methods： The model of UC rat was made by TNBS,whole-cell protein was extracted from colonic mucosa.Western blot technique was used to detect protein levels of pERK1/2 and pMEK1/2.Results： Compared with normal group,pMEK1/2 and pERK1/2 expressionof model group increased slightly in the third day,and pMEK1/2 and pERK1/2 expression continued to heighten in the 7th day（P〈0.05,P〈0.01,P〉0.05）.In the 14th day,these two proteins expression increased significantly（P〈0.05,P〈0.01,P〈0.05）.Compared with model group,the relative protein expressions of pMEK1/2 and pERK1/2 of treatment group increased slightly in the third day,and then heighten obviously in the 7th and 14th day（P〈0.05,P〈0.05,P〈0.01）.Conclusion： The relative protein expressions of pMEK1/2 and pERK1/2 in colonic mucosa of UC model rats induced by TNBS were enhanced after using kuijieling decoction.MEK/ERK message pathway may be one of pathways to protect and repair intestinal mucosal injury in UC rats treated by Kuijieling decoction.

关 键 词：溃结灵 溃疡性结肠炎 磷酸化MEK1/2 磷酸化ERK1/2 

分 类 号：R285.5[医药卫生—中药学]