IFN-α治疗慢性乙型肝炎疗效与记忆性T细胞的相关性  

Influence of interferon-α therapy on CD8 T memory subsets in patients with chronic hepatitis B

作　　者：刘道洁[1] 董培玲[1] 丁惠国[1] 赵敬敬[1] 李娟[1] 孟庆华[1] 闫丽[1] 孙斌[1] 武彦宁[1] 王延军[1] 

机构地区：[1]北京市卫生局肝炎研究所,首都医科大学附属北京佑安医院肝炎研究所,100069

出　　处：《中华微生物学和免疫学杂志》2011年第3期265-268,共4页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金资助项目（30600553）;2008年北京市优秀人才培养资助个人项目（20081D0301600099）

摘　　要：目的 研究IFN-α在治疗慢性乙型肝炎患者中对记忆性T细胞亚群变化的影响,及其临床治疗效果与记忆性T细胞亚群变化的相关性.方法 57例接受IFN-α治疗的慢性乙型肝炎（CHB）患者,分别在治疗前（O周）,及疗程期内不同时间点（12周和24周）抽取外周血,分离外周血单个核细胞（PBMCs）后加入抗体染色,流式细胞仪检测,统计学分析.结果 非活动性HBsAg携带者CD8效应记忆性T细胞（CD8＋TEM）的比例显著高于未经IFN-α治疗的CHB患者;相反,CD8中心记忆性T细胞（CD8＋TCM）的百分比则显著低于未经IFN-α治疗的CHB患者（P〈0.05）;有效组CD8＋TEM百分比在IFN-α治疗前和治疗24周时均高于无效组,CD8＋TCM百分比在IFN-α治疗前和治疗24周时则低于无效组（P〈0.05）;有效组IFN-α的剂量明显高于无效组.结论 病毒的清除与效应记忆性T细胞亚群比例可能有关,TEM亚群在数量上占优势可能对IFN-α治疗效果有益.Objective To investigate the influence of interferon-a therapy on CD8 T memory subsets in patients with chronic hepatitis B（CHB） and correlation between the effect of IFN-α and CD8 T memory subsets. Methods Blood samples from 57 patients with CHB were collected before treatment （0 week）, at 12 weeks and 24 weeks of treatment with pegylated IFN-α. Assays were performed on freshly isolated peripheral blood mononuclear cells （ PBMCs）. For phenotype analysis, All data were acquired on a flow cytometer instrument and prepared for analysis. Results A significantly higher frequency of CD8＋ TEM and lower frequency of CD8＋ TCM in inactive HBsAg carriers than that in CHB patients prior to treatment was observed （P 〈0.05）. The proportion of CD8 ＋ TCM was higher in group nonresponders than in group respond-ers, and the proportion of CD8 ＋ TEM was lower in group nonresponders than in group responders （P 〈 0.05 ）. The average dosage of IFN-α applied to patients with response was significantly higher than nonresponders. Conclusion The dominance of circulating effector memory T cells may be associated with elimination of viral infection, and possibly benefit for response to therapy with IFN-α.

关 键 词：记忆性T细胞 慢性乙型肝炎 IFN—α 

分 类 号：R512.62[医药卫生—内科学]