慢性囊型包虫病患者外周血单个核细胞中TLR2、4、7mRNA表达水平的研究  

The expression of TLR2, 4, 7 mRNA on peripheral blood mononuclear cells in patients with chronic cystic echinococcosis infection

作　　者：单骄宇[1,2] 吐尔洪江·吐逊[1] 王俊华[1] 张志[1] 李亮[1] 张雪[1] 张传山[1] 刘涛[1] 林仁勇[1] 温浩[1] 

机构地区：[1]新疆医科大学第一附属医院新疆包虫病基础医学重点实验室,乌鲁木齐830054 [2]新疆医科大学基础医学院人体寄生虫学教研室

出　　处：《中华微生物学和免疫学杂志》2011年第3期273-278,共6页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金项目（30800967,30960342）;自治区自然科学基金项目（2008211）;新疆包虫病基础医学重点实验室开放课题资助项目（XJDX0202-2008-05）

摘　　要：目的 探讨慢性囊型包虫病（CE）患者外周血单个核细胞（PBMC）Toll样受体（TLRs）2、4、7基因表达变化及血清IL-10的表达情况.方法 采用实时荧光定量PCR（FQ-RT-PCR）法检测42例慢性CE患者与28例健康对照组（NC）外周血中TLR2、4、7 mRNA的表达,GAPDH作为内参基因;应用ELISA方法检测两组血清中IL-10的浓度.t检验比较两组间TLR2、4、7及IL-10的表达差异;TLR2、4、7的表达水平间及它们与血清IL-10水平间的相关性分析采用线性相关分析.结果 慢性CE组的TLR2、TLR4、TLR7的mRNA相对表达量均比NC组的表达增高,其中CE组的TLR2是NC组的7.3倍,TLR4是NC组的3.6倍,TLR7是NC组的3.6倍.CE组的TLR2、TLR4、TLR7 mRNA相对表达量分别是1.0729±0.4006、5.0976±1.6682、0.6481±0.2574;NC组的TLR2、TLR4、TLR7 mRNA的相对表达量分别是0.1468±0.0435、1.4067 ±0.3279、0.1804±0.0568.与NC组相比,CE组的TLR2、TLR4的差异均具有统计学意义（P值分别为0.0287、0.033）,而TLR7则无统计学意义（P=0.0862）.慢性CE组、NC组外周血清IL-10分别为（17.6770±1.6298）pg/ml、（9.4898±0.7049）pg/ml,且慢性CE组与NC组的IL-10比较,差异具有统计学意义（P=0.0002）;在慢性CE组中,外周血PBMC的TLR2与TLR4在mRNA相对表达水平上呈正相关（r=0.1135,P=0.036）,其他均无相关.结论 TLR2、TLR4参与了慢性囊型包虫病的发生发展过程,TLR2、TLR4可作为囊型包虫抗原的受体,与不同抗原成分相互作用,调节宿主的免疫应答,同时在血清IL-10分泌增高的作用下共同促使机体向Th2型免疫应答方向发展,从而对囊型包虫感染所产生的免疫逃避发挥着重要作用.Objective To explore the expression of TLR2,4,7 mRNA on peripheral blood mono-nuclear cells （PBMCs） in patients with chronic cystic echinococcosis（CE） infection, and the level of serum IL-10. Methods The expression level of TLR2,4,7 mRNA on peripheral blood mononuclear were tested in 42 chronic CE cases and 28 normal controls （NC） by real-time fluorescent quantitative reverse transcription polymerase chain reaction （FQ-RT-PCR） method. GAPDH was selected as the internal control. The level of serum IL-10 was determined in ELISA. The subjects were determined by t test. The correlations between TLR2, TLR4, TLR7 and IL-10 were determined by differences of expression of TLR2, TLR4, TLR7 on PBMCs and serum IL-10 in two groups of study linear correlation test. Results The expressions of TLR2, TLR4,TLR7 mRNA in chronic CE group were higher than those of in NC group. Compared with the NC group, the expressions of TLR2, TLR4 and TLR7 mRNA increased more than 7.3-, 3.6-, 3.6-fold, respectively. In chronic CE group, TLR2, TLR4 and TLR7 mRNA expressions were 1.0729 ±0.4006, 5.0976 ±1.6682, 0. 6481 ±0. 2574, respectively. TLR2, TLR4 and TLR7 mRNA expressions were 0. 1468 ± 0.0435, 1.4067 ±0. 3279, 0. 1804 ±0. 0568 in NC group, respectively. Compared with NC group, the differences of TLR2 and TLR4 mRNA expression were significant （P = 0.0287, 0. 033）, while the expression of TLR7 mRNA was not difference （P =0.0862）. Moreover, in chronic CE group, the level of serum IL-10 was higher than that of in NC group. In chronic CE group and NC group, the level of serum IL-10 was （17.6770±1.6298） pg/ml, （9.4898 ±0.7049） pg/ml. Compared with NC group, there was significant difference in chronic group （P〈0.01）. Significant positive correlation between TLR2 and TLR4 was found in chronic CE group, r = 0. 1135, P =0.036. Others were not correlations. Conclusion In the development of chronic CE, TLR2 and TLR4 participate in this progression. As the receptors of antigen of cystic echinococcus, TLR2 an

关 键 词：慢性囊型包虫病 TOLL样受体 外周血单个核细胞 转录水平 

分 类 号：R532.32[医药卫生—内科学]