小菜蛾高效Bt菌株的分离、生化特性及基因型鉴定  

Isolation,biochemical and cry-type gene characterization of Bacillus thuringiensis strains with high toxic to Plutella xylostella

作　　者：朱勋[1,2] 吴青君[2] 张友军[2] 刘春光[1] 刘岩林[1] 余亚军[1] 景伟[1] 薛原[1] 杨峰山[1] 

机构地区：[1]黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,哈尔滨150080 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《应用昆虫学报》2011年第2期285-290,共6页Chinese Journal of Applied Entomology

基　　金：公益性行业(农业)科研专项(200803001);国家自然科学基金(30700539);黑龙江省自然科学基金(C2007-03);哈尔滨市科技创新人才研究专项(2007RFXXN048);黑龙江省教育厅面上项目(11511273)

摘　　要：从哈尔滨田间采集死亡小菜蛾Plutella xylostella(L.)幼虫,从中分离出10株苏云金芽孢杆菌Bacillus thuringiensis。对小菜蛾幼虫室内生物测定结果表明,各菌株对小菜蛾幼虫的死亡率均在85%以上,其中DBW902毒力最强,48h的LC50为13.99mg/L。菌株cry/cyt基因检测表明,所有菌株均含有cry1A或cry2A基因,这与其高毒力的特性基本吻合。生化检测与分析表明,菌株DBW904、DBW93、DBW962与已报道的苏云金芽孢杆菌山东亚种B.thuringiensis subsp.shandongiensis的生化特性一致,但是其它菌株的生理生化特性与已报道菌株有区别。The insecticidal activities of ten strains of Bacillus thuringiensis isolated from diseased Plutella xylostella（L.） larvae were characterized.All strains caused 80% larval mortality and the strain DBW902 had the highest insecticidal activity with an LC50 of 13.99 mg/L at 48 h.The biochemical characteristics and cry/cyt genes of each strain were determined.High insecticidal activity coincided with the detection of at least one cry1A or cry2A family gene.The biochemical characteristics of the strains,DBW904,DBW93 and DBW962 were homologous to B.thuringiensis subsp.and Shandongiensis,but not the other strains.The identification of these 10 B.thuringiensis isolates with moderate to high insecticidal activity against P.xylostella is promising for the control of this pest in northeastern China.

关 键 词：苏云金芽孢杆菌 鉴定 杀虫活性 cry/cyt基因 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治] S476.12[农业科学—植物保护]