橙皮苷抗大鼠肝纤维化作用的实验研究  

Protective effect of hesperidin on experimental liver fibrosis in rats

作　　者：吴芙蓉[1] 李俊[1] 任丹阳[1] 陶辉[1] 贾丽莎[1] 

机构地区：[1]安徽医科大学药学院,安徽天然药物活性研究省级重点实验室,国家中医药管理局中医药科研三级实验室“中药药理实验室”,合肥230032

出　　处：《安徽医科大学学报》2011年第4期358-361,共4页Acta Universitatis Medicinalis Anhui

摘　　要：目的观察橙皮苷(HDN)抗大鼠肝纤维化作用。方法 60只SD大鼠,随机分为正常组、模型组、HDN(50、100、200 mg/kg)3个剂量组和秋水仙碱(0.1 mg/kg)组,每组10只。除正常组外,其余各组采用50%四氯化碳(CCl4)皮下注射,每周2次,连续12周诱导肝纤维化大鼠模型。于造模第7周起,治疗组分别灌胃给予HDN(50、100、200 mg/kg)、秋水仙碱(0.1 mg/kg),正常组和模型组给予等容量生理盐水,共6周。实验结束后,股动脉采血,测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原氨端肽(PⅢNP)、Ⅳ型胶原(CⅣ)、转化生长因子(TGF)-β1和肝匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量;肝组织HE染色,RT-PCR法测定肝组织中TGF-β1 mRNA的表达。结果 HDN各剂量组均能明显降低肝纤维化大鼠血清中ALT、AST、HA、LN、PⅢNP、CIV、TGF-β1和肝组织中MDA含量,升高SOD水平,抑制肝组织中TGF-β1 mRNA的表达;同时,病理组织学检查结果显示HDN各剂量组大鼠肝纤维化增生程度明显减轻。结论 HDN对CCl4诱导的肝纤维化大鼠有一定的保护作用,其机制可能与抗机体脂质过氧化、调节TGF-β1的产生有关。Objective To investigate the anti-liver fibrosis effect of hesperidin（HDN） in rats.Methods Rat liver fibrosis was induced by 50% CCl4 twice a week for 12 weeks.From the 7th week,all the therapeutic groups were treated with the HDN（50,100,200 mg/kg）and colchicine（0.1 mg/kg）respectively for 6 weeks.The levels of ALT,AST,HA,LN,PⅢNP,CIV,TGF-β1,MDA and SOD were measured.HE staining was used to evaluate the degree of hepatic fibrosis.The mRNA expression of TGF-β1 in liver tissue was detected by RT-PCR.Results HDN（50,100,200 mg/kg）not only reduced the levels of ALT,AST,HA,LN,PⅢNP,CIV,MDA,TGF-β1 significantly and the degree of hepatic fibrosis,but also increased SOD activity.Moreover,HDN decreased the mRNA expression of TGF-β1 in liver tissues obviously.Conclusion HDN has anti-liver fibrosis effect,which may be associated with its free-radical scavenging ability and TGF-β1 regulation.

关 键 词：肝硬化 橙皮苷 大鼠 转化生长因子Β1 脂质过氧化作用 

分 类 号：R287[医药卫生—中药学] R341[医药卫生—中医学]