白介素22及其相关因子在银屑病患者血清及外周血单一核细胞中的表达  

Expressions of interleukin （IL）-22 and related cytokines in the sera and peripheral blood mononuclear cells （PBMCs） of patients with psoriasis

作　　者：史玉玲[2] 徐晓光[1] 毕新岭[1] 王英[1] 卜晓琳[1] 顾军[1] 

机构地区：[1]第二军医大学附属长海医院皮肤科,上海200433 [2]同济大学附属第十人民医院皮肤科,上海200072

出　　处：《中华皮肤科杂志》2011年第4期238-240,共3页Chinese Journal of Dermatology

基　　金：上海市科学技术委员会基金（09411960600）

摘　　要：目的 探讨白介素（IL）-22及其相关因子IL-23p19、IL-6在寻常性银屑病患者外周血单一核细胞（PBMC）及血清中表达的意义.方法 采用实时定量RT-PCR法检测寻常性银屑病患者及正常人对照者外周血PBMC中IL-22 mRNA、IL-23p19 mRNA及IL-6 mRNA的水平;采用ELISA法检测寻常性银屑病患者及正常人对照者血清和PBMC培养上清中IL-22的分泌水平.结果 银屑病组和正常人对照组PBMC中IL-22 mRNA的相对表达量分别为4.48 4±2.64和2.35±0.91;IL-23p19 mRNA的相对表达量分别为6.07±4.09和2.61 4±1.46;IL-6 mRNA的相对表达量分别为3.87 4±1.49和1.48 4±0.62;差异均有统计学意义（P〈0.01）.ELISA检测发现,银屑病组和正常人对照组血清中IL-22的水平分别为（86.23 ±25.58）ng/L和（43.67±14.82）ng/L（P〈0.01）,PBMC培养上清中IL-22的水平分别为（119.11±21.51）ng/L和（57.70±13.17）ng/L（P〈0.01）.结论 寻常性银屑病患者PBMC和血清中过度表达IL-22,提示IL-22可能参与银屑病的发病机制.Objective To investigate the expressions and significance of IL-22 and related cytokines （IL-23pl9 and IL-6） in sera and PBMCs of patients with psoriasis. Methods Sera and PBMCs were obtained from the venous blood samples from 58 patients with psoriasis vulgaris and 20 normal human controls. The PBMCs were subjected to culture for 5 hours followed by the collection of cells and culture supernatant. Then,quantitative real-time RT-PCR was used to examine the mRNA expressions of IL-22, IL-23pl9 and IL-6 in PBMCs, enzyme-linked immunosorbent assay （ELJSA） to detect the level of IL-22 protein in the sera and culture supernatant of PBMCs. Results In the patients with psoriasis and controls, the relative expression level in PBMCs was 4.48 ± 2.64 and 2.35 ± 0.91 respectively for IL-22 mRNA, 6.07 ± 4.09 and 2.61 ± 1.46 respectively for IL-23pl9 mRNA, 3.87 ± 1.49 and 1.48 ± 0.62 respectively for IL-6 mRNA; significant differences were observed between the two groups in all the above parameters （all P 〈 0.01）. ELISA revealed that the level of IL-22 protein in the patients and controls was （86.23 ± 25.58） ng/L and （43.67 ± 14.82） ng/L respectively in the sera （P〈 0.01）, （119.11 ± 21.51） ng/L and （57.70 ± 13.17） ng/L respectively in the culture supernatant of PBMCs （P〈 0.01）. Conclusion There is an overexpression of IL-22 in the PBMCs and sera of patients with psoriasis, implying that IL-22 is involved in the pathogenesis of psoriasis.

关 键 词：银屑病 白细胞介素22 白细胞介素23p19 白细胞介素6 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]