地塞米松对系统性红斑狼疮阴虚内热证患者外周血单一核细胞基因表达谱的影响  

Influence of dexam ethasone on the gene expression profiling of peripheral blood mononuclear cells （PBMCs） from patients with internal heat due to Yin deficiency-type SLE

作　　者：程喜平[1] 范瑞强[2] 赖梅生[3] 陈达灿[2] 禤国维[2] 朱晓浚[4] 陈明春[4] 

机构地区：[1]广州医学院第一附属医院皮肤科,510120 [2]广东省中医院皮肤科 [3]南方医科大学中医学院中医外科教研室 [4]中山大学附属第二医院皮肤科

出　　处：《中华皮肤科杂志》2011年第4期280-282,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30472218）

摘　　要：目的 探讨地塞米松对系统性红斑狼疮（SLE）阴虚内热证患者外周血单一核细胞（PBMC）基因表达谱的影响.方法 用地塞米松孵化SLE阴虚内热证患者PBMC,收集孵化后细胞进行PBMC的基因表达谱检测.结果 地塞米松上调85个PBMC基因,下调126个PBMC基因.结论 地塞米松调控SLE阴虚内热证患者PBMC的基因所属功能簇众多,基因编码的功能蛋白分布细胞多个部位,不是所有基因调控结果均有利于SLE缓解,一些基因调控作用对研究地塞米松治疗SLE的机制有帮助.Objective To observe the influence of dexamethasone on the gene expression profiling of PBMCs from patients with internal heat due to Yin deficiency-type SLE. Methods The PBMCs from 3 patients with internal heat due to Yin deficiency-type SLE were incubated with dexamethasone of 10-6 g/L for 48 hours followed by the detection of gene expression profiling of PBMCs by a GeneChip. Results The incubation with dexamethasone upregulated the expressions of 85 genes and downregulated those of 126 genes in PBMCs from the SLE patients. Conclusions Dexamethasone can regulate the expressions of numerous clusters of genes in PBMCs from patients with internal heat due to Yin deficiency-type SLE, and functional proteins encoded by these genes are located at various organelles and regions of cells. Not all the results of gene regulation by dexamethasone favor the relief of SLE. To study the regulation of genes may be beneficial to the elucidation of mechanisms underlying the therapeutic effect of dexamethasone on SLE.

关 键 词：外周血单一核细胞 系统性红斑狼疮 基因表达谱 阴虚内热证 地塞米松 患者 PBMC 基因调控 

分 类 号：R593.241[医药卫生—内科学]